Clone: 35033876546138376 F08
Tagged gene: CG5514
Identifier: FBgn0039560
| Clone | FlyFos024854(pRedFlp-Hgr)(CG5514[27178]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation(click to hide details)
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad | |
| Whole tag sequencing | flipped | download [show/hide details] |
GENOME<<>>TAG TAG<<>>GENOME
PREDICTED TCTGTTCTCATGCTCATCCTCAATATTCTTCTTTACCGCCTCTATTTTCTCCCCCACACGCATCCGCATTGTTAACTAATATACATAATATGTAGCTGTAGAAGTGCATACCAATCAGGACCCGCTGGACGAGGTTCACACAAACCAAGATCCACTTGATGAATTCATGGTGTCCAAGGGCGAGGAGCTGTTCACCGGCGTGGTGCCCATCCTGGTGGAGCTGGATGGCGACGTGAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGACGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTGGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGATCACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTGAAGCCACCACTAGTAGTCCTAACCAAACCGCAATGGAGCTGGGTAAGCGGTTCCCGCGCGATTCTCTGTCCTTTGCCCGCCGATTGTTGATGCTTCGA
COVERAGE 00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111222222222222222222222222222222222222111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111
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EV5 CACATGAAGCAGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAGGAGCGCACCATCAGCTTCAAGGATGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGATACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGATTTCAAGGAGGATGGCAACATCCTGGGCCACAAGCTGGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGATAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAATGTGGAGGATGGCTCCGTGCAGCTGGCCGATCACTACCAGCAGAACACCCCCATCGGCGACGGCCCAGTGCTGCTGCCCGATAACCACTACCTGAGCACCCAGAGCGTGCTGTCCAAGGACCCCAACGAGAAGCGCGATCACATGGTGCTGCTGGAGTTCGTGACCGCCGCCGGCATCACCCTGGGCATGGATGAGCTGTACAAGCTCGAGGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGATAGCACCCTGGAGGTGCTGTTCCAGGGCCCCGAGAACCTGTACTTCCAGGGCATGGCCAGCAGCCTGCGCCAGATCCTGGATAGCCAGAAGATGGAGTGGCGCAGCAACGCCGGCGGCAGCGGATCCTCGGGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGT
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DS88 GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGTCGACAGATTATAAAGACCACGATGGAGACTATAAAGATCATGACATTGACTACAAGGATGACGACGACAAGTGAAGCCACCACTAGTAGTCCTAACCAAACCGCAATGGAGCTGGGTAAGCGGTTCCCGCGCGATTCTCTGTCCTTTGCCCGCCGATTGTTGATGCTTCGA
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Genes in pFlyFos vector(click to hide details)
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-3R | ||||||||
| 23783910 | 23784967 | 10373 | 11430 | + | gene | CG42487 | coding_transcript | |
| 23783106 | 23784307 | 11033 | 12234 | + | gene | CG4884 | coding_transcript | |
| 23780639 | 23783048 | 12292 | 14701 | - | gene | CG5527 | coding_transcript | |
| 23777773 | 23779834 | 15506 | 17567 | + | gene | CG4951 | coding_transcript | |
| 23774380 | 23777882 | 17458 | 20960 | - | gene | Gp93 | coding_transcript | |
| 23768511 | 23773731 | 21609 | 26829 | + | gene | mfrn | coding_transcript | |
| 23763967 | 23768500 | 26840 | 32435 | - | gene | CG5514 | coding_transcript | |
| 23763967 | 23768500 | 26840 | 32435 | - | mrna | FBtr0085252 | coding_transcript | |
| 23763967 | 23768500 | 26840 | 32435 | - | mrna | FBtr0085253 | coding_transcript | |
| 23763967 | 23768500 | 26840 | 32435 | - | mrna | FBtr0301143 | coding_transcript | |
| 23768157 | 23768500 | 26840 | 28245 | - | exon | coding_transcript | ||
| 23768165 | 23768500 | 26840 | 27175 | - | three_prime_utr | coding_transcript | ||
| 23768310 | 23768500 | 26840 | 27030 | - | exon | coding_transcript | ||
| 23768310 | 23768500 | 26840 | 27030 | - | three_prime_utr | coding_transcript | ||
| 23768310 | 23768500 | 26840 | 27030 | - | exon | coding_transcript | ||
| 23768441 | 23768500 | 26840 | 26899 | - | three_prime_utr | coding_transcript | ||
| 23768310 | 23768440 | 26900 | 27030 | - | cds | coding_transcript | ||
| 23767856 | 23768309 | 27031 | 28546 | - | intron | coding_transcript | ||
| 23768206 | 23768309 | 27031 | 27134 | - | intron | coding_transcript | ||
| 23768157 | 23768205 | 27135 | 28245 | - | exon | coding_transcript | ||
| 23768165 | 23768205 | 27135 | 27175 | - | three_prime_utr | coding_transcript | ||
| 23768157 | 23768164 | 27176 | 28245 | - | cds | coding_transcript | ||
| 23768157 | 23768164 | 27176 | 28245 | - | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 2437 | 2470 | 27252 | 27285 | - | misc_recomb | FRT | CLC | |
| 2358 | 2429 | 27293 | 27364 | - | cds | BLRP | CLC | |
| 2337 | 2357 | 27365 | 27385 | - | cds | TEV | CLC | |
| 2313 | 2336 | 27386 | 27409 | - | cds | Precision cut site | CLC | |
| 2271 | 2312 | 27410 | 27451 | - | cds | V5 | CLC | |
| 1548 | 2264 | 27458 | 28174 | - | cds | SGFP | CLC | |
| 1482 | 1541 | 28181 | 28240 | - | cds | 2xTY1 | CLC | |
| dmel-5.43-3R | ||||||||
| 23767856 | 23768156 | 28246 | 28546 | - | intron | coding_transcript | ||
| 23767856 | 23768156 | 28246 | 28546 | - | intron | coding_transcript | ||
| 23765429 | 23767855 | 28547 | 30973 | - | exon | coding_transcript | ||
| 23765429 | 23767855 | 28547 | 30973 | - | cds | coding_transcript | ||
| 23765429 | 23767855 | 28547 | 30973 | - | exon | coding_transcript | ||
| 23765429 | 23767855 | 28547 | 30973 | - | cds | coding_transcript | ||
| 23765429 | 23767855 | 28547 | 30973 | - | exon | coding_transcript | ||
| 23765429 | 23767855 | 28547 | 30973 | - | cds | coding_transcript | ||
| 23765370 | 23765428 | 30974 | 31032 | - | intron | coding_transcript | ||
| 23765370 | 23765428 | 30974 | 31032 | - | intron | coding_transcript | ||
| 23765370 | 23765428 | 30974 | 31032 | - | intron | coding_transcript | ||
| 23764699 | 23765369 | 31033 | 31703 | - | exon | coding_transcript | ||
| 23764699 | 23765369 | 31033 | 31703 | - | cds | coding_transcript | ||
| 23764699 | 23765369 | 31033 | 31703 | - | exon | coding_transcript | ||
| 23764699 | 23765369 | 31033 | 31703 | - | cds | coding_transcript | ||
| 23764699 | 23765369 | 31033 | 31703 | - | exon | coding_transcript | ||
| 23764699 | 23765369 | 31033 | 31703 | - | cds | coding_transcript | ||
| 23764641 | 23764698 | 31704 | 31761 | - | intron | coding_transcript | ||
| 23764641 | 23764698 | 31704 | 31761 | - | intron | coding_transcript | ||
| 23764641 | 23764698 | 31704 | 31761 | - | intron | coding_transcript | ||
| 23764166 | 23764640 | 31762 | 32236 | - | exon | coding_transcript | ||
| 23764166 | 23764640 | 31762 | 32236 | - | exon | coding_transcript | ||
| 23764166 | 23764640 | 31762 | 32236 | - | exon | coding_transcript | ||
| 23764417 | 23764640 | 31762 | 31985 | - | cds | coding_transcript | ||
| 23764417 | 23764640 | 31762 | 31985 | - | cds | coding_transcript | ||
| 23764417 | 23764640 | 31762 | 31985 | - | cds | coding_transcript | ||
| 23764166 | 23764416 | 31986 | 32236 | - | five_prime_utr | coding_transcript | ||
| 23764166 | 23764416 | 31986 | 32236 | - | five_prime_utr | coding_transcript | ||
| 23764166 | 23764416 | 31986 | 32236 | - | five_prime_utr | coding_transcript | ||
| 23764104 | 23764165 | 32237 | 32298 | - | intron | coding_transcript | ||
| 23764104 | 23764165 | 32237 | 32298 | - | intron | coding_transcript | ||
| 23764104 | 23764165 | 32237 | 32298 | - | intron | coding_transcript | ||
| 23763967 | 23764103 | 32299 | 32435 | - | exon | coding_transcript | ||
| 23763967 | 23764103 | 32299 | 32435 | - | five_prime_utr | coding_transcript | ||
| 23763967 | 23764103 | 32299 | 32435 | - | exon | coding_transcript | ||
| 23763967 | 23764103 | 32299 | 32435 | - | five_prime_utr | coding_transcript | ||
| 23763967 | 23764103 | 32299 | 32435 | - | exon | coding_transcript | ||
| 23763967 | 23764103 | 32299 | 32435 | - | five_prime_utr | coding_transcript | ||
| 23758704 | 23763571 | 32831 | 37698 | + | gene | Mes-4 | coding_transcript | |
Sets(click to hide details)
Oligos(click to hide details)
| Name/Sequence | Export |
|---|---|
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CG5514[27178]::S000169_fly_pretag_fwd
CCCCCACACGCATCCGCATTGTTAACTAATATACATAATATGTAGCTGTAgaagtgcataccaatcaggacccgc
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Fasta
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CG5514[27178]::S000169_fly_pretag_rev
GCTTACCCAGCTCCATTGCGGTTTGGTTAGGACTACTAGTGGTGGCTTCActtgtcgtcgtcatccttgtagtca
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Fasta
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