Clone: 35033876546138376 E07

Tagged gene: Gem3
Identifier: FBgn0011802

Clone FlyFos024672(pRedFlp-Hgr)(Gem3[21720]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad
Whole tag sequencing flipped download [show/hide details]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
10452721 10456199 21457 25997 - gene Gem3 coding_transcript
10452721 10456199 21457 25997 - mrna FBtr0076292 coding_transcript
10452721 10455878 21457 25676 - exon coding_transcript
10452721 10452981 21457 21717 - three_prime_utr coding_transcript
10452982 10455878 21718 25676 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 21794 21827 - misc_recomb FRT CLC
2358 2429 21835 21906 - cds BLRP CLC
2337 2357 21907 21927 - cds TEV CLC
2313 2336 21928 21951 - cds Precision cut site CLC
2271 2312 21952 21993 - cds V5 CLC
1548 2264 22000 22716 - cds SGFP CLC
1482 1541 22723 22782 - cds 2xTY1 CLC
dmel-5.43-3L
10455879 10455936 25677 25734 - intron coding_transcript
10455937 10456199 25735 25997 - exon coding_transcript
10455937 10456126 25735 25924 - cds coding_transcript
10456127 10456199 25925 25997 - five_prime_utr coding_transcript
10456916 10457735 26714 27533 + gene CG32061 coding_transcript
10458287 10461580 28085 31378 + gene S-Lap3 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Gem3[21720]::S000169_fly_pretag_fwd
AGTACCAATTCATTGCATCCCATGTGGCCAAGAATCTACAGAACTATTACgaagtgcataccaatcaggacccgc
 fasta Fasta
Gem3[21720]::S000169_fly_pretag_rev
TTTAATTTTAATGCCTGAGTTTTCACTAGTTTTTCATTCATAACCACTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta