Clone: 35033876546138376 H06

Tagged gene: x16
Identifier: FBgn0028554

Clone FlyFos024565(pRedFlp-Hgr)(x16[17980]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
6906918 6907834 9853 10769 - gene CG13773 coding_transcript
6908087 6911432 11022 14367 + gene Rat1 coding_transcript
6911338 6914137 14273 17072 - gene wee coding_transcript
6914300 6920274 17235 24271 - gene x16 coding_transcript
6914300 6920274 17235 24271 - mrna FBtr0079368 coding_transcript
6914300 6915400 17235 19397 - exon coding_transcript
6914300 6915042 17235 17977 - three_prime_utr coding_transcript
6915043 6915400 17978 19397 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 18054 18087 - misc_recomb FRT CLC
2358 2429 18095 18166 - cds BLRP CLC
2337 2357 18167 18187 - cds TEV CLC
2313 2336 18188 18211 - cds Precision cut site CLC
2271 2312 18212 18253 - cds V5 CLC
1548 2264 18260 18976 - cds SGFP CLC
1482 1541 18983 19042 - cds 2xTY1 CLC
dmel-5.43-2L
6915401 6919523 19398 23520 - intron coding_transcript
6915683 6916387 19680 20384 - gene CG32829 coding_transcript
6915683 6916387 19680 20384 - mrna FBtr0079370 coding_transcript
6915683 6916387 19680 20384 - exon coding_transcript
6915683 6916387 19680 20384 - cds coding_transcript
6916753 6918741 20750 22738 - gene nop5 coding_transcript
6916753 6918741 20750 22738 - mrna FBtr0079369 coding_transcript
6916753 6917162 20750 21159 - exon coding_transcript
6916753 6916897 20750 20894 - three_prime_utr coding_transcript
6916898 6917162 20895 21159 - cds coding_transcript
6917163 6917340 21160 21337 - intron coding_transcript
6917341 6918741 21338 22738 - exon coding_transcript
6917341 6918611 21338 22608 - cds coding_transcript
6918612 6918741 22609 22738 - five_prime_utr coding_transcript
6919524 6920274 23521 24271 - exon coding_transcript
6919524 6919942 23521 23939 - cds coding_transcript
6919943 6920274 23940 24271 - five_prime_utr coding_transcript
6920693 6928678 24690 32675 + gene Hrb27C coding_transcript
6932244 6932552 36241 36549 + gene CG43232 coding_transcript
6932685 6942814 36682 46811 - gene CG18304 coding_transcript
6943740 6945284 47737 49281 - gene CG10158 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
x16[17980]::S000169_fly_pretag_rev
GCCCTCATCATCGCATGTCGGGCTCCGTCTGCATCCGTTCGCTGTCGCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
x16[17980]::S000169_fly_pretag_fwd
CGCCCAGGCGTCGTGGTGACTCGTCCGCCTCACGATCCGTTTCAAGGGACgaagtgcataccaatcaggacccgc
 fasta Fasta