Clone: 35033876546138376 D04

Tagged gene: wol
Identifier: FBgn0261020

Clone FlyFos023995(pRedFlp-Hgr)(wol[37123]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad
Whole tag sequencing flipped download [show/hide details]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
8321276 8325299 13364 17387 + gene CG7781 coding_transcript
8320324 8321285 17378 18339 - gene CG7787 coding_transcript
8316889 8319920 18743 21774 + gene mtsh coding_transcript
8311419 8317017 21646 27244 - gene CG7806 coding_transcript
8309869 8311144 27519 28794 + gene CG7810 coding_transcript
8308440 8309963 28700 30223 - gene CG7818 coding_transcript
8306684 8308188 30475 31979 + gene CG7830 coding_transcript
8305590 8306743 31920 33073 - gene CG7840 coding_transcript
8303433 8305376 33287 35230 - gene Scgalpha coding_transcript
8301345 8302983 35680 38380 + gene wol coding_transcript
8301345 8302983 35680 38380 + mrna FBtr0079614 coding_transcript
8302758 8302983 35680 35905 + exon coding_transcript
8302824 8302983 35680 35839 + five_prime_utr coding_transcript
8302758 8302823 35840 35905 + cds coding_transcript
8302699 8302757 35906 35964 + intron coding_transcript
8302524 8302698 35965 36139 + exon coding_transcript
8302524 8302698 35965 36139 + cds coding_transcript
8302333 8302523 36140 36330 + intron coding_transcript
8301936 8302332 36331 36727 + exon coding_transcript
8301936 8302332 36331 36727 + cds coding_transcript
8301880 8301935 36728 36783 + intron coding_transcript
8301345 8301879 36784 38380 + exon coding_transcript
8301537 8301879 36784 38188 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 37124 37183 + cds 2xTY1 CLC
1548 2264 37190 37906 + cds SGFP CLC
2271 2312 37913 37954 + cds V5 CLC
2313 2336 37955 37978 + cds Precision cut site CLC
2337 2357 37979 37999 + cds TEV CLC
2358 2429 38000 38071 + cds BLRP CLC
3813 3846 38079 38112 + misc_recomb FRT CLC
dmel-5.43-2L
8301345 8301536 38189 38380 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
wol[37123]::S000169_fly_pretag_rev
ACAAAATGCTAGTTAATATTGGGAATGAGATGCCCTCCAAGTCCACATCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
wol[37123]::S000169_fly_pretag_fwd
GATATTTGGTGGGCGCCTGGCGGATTGCCTCCATACAGAAGAAGGAAAAGgaagtgcataccaatcaggacccgc
 fasta Fasta