Clone: 35033876546138376 E01

Tagged gene: CG4300
Identifier: FBgn0036272

Clone FlyFos023570(pRedFlp-Hgr)(CG4300[24720]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
12260645 12262903 19805 22063 + gene CG32100 coding_transcript
12263068 12264453 22228 23613 - gene Pbgs coding_transcript
12264753 12270366 23913 30588 - gene CG4300 coding_transcript
12264753 12270366 23913 30588 - mrna FBtr0076009 coding_transcript
12264753 12270366 23913 30588 - mrna FBtr0076010 coding_transcript
12264753 12266141 23913 26363 - exon coding_transcript
12264753 12266141 23913 26363 - exon coding_transcript
12264753 12265557 23913 24717 - three_prime_utr coding_transcript
12264753 12265557 23913 24717 - three_prime_utr coding_transcript
12265558 12266141 24718 26363 - cds coding_transcript
12265558 12266141 24718 26363 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 24794 24827 - misc_recomb FRT CLC
2358 2429 24835 24906 - cds BLRP CLC
2337 2357 24907 24927 - cds TEV CLC
2313 2336 24928 24951 - cds Precision cut site CLC
2271 2312 24952 24993 - cds V5 CLC
1548 2264 25000 25716 - cds SGFP CLC
1482 1541 25723 25782 - cds 2xTY1 CLC
dmel-5.43-3L
12266142 12266597 26364 26819 - intron coding_transcript
12266142 12266597 26364 26819 - intron coding_transcript
12266598 12266720 26820 26942 - exon coding_transcript
12266598 12266720 26820 26942 - cds coding_transcript
12266598 12266720 26820 26942 - exon coding_transcript
12266598 12266720 26820 26942 - cds coding_transcript
12266721 12268175 26943 28397 - intron coding_transcript
12266721 12267102 26943 27324 - intron coding_transcript
12267103 12267211 27325 27433 - exon coding_transcript
12267103 12267211 27325 27433 - cds coding_transcript
12267212 12269989 27434 30211 - intron coding_transcript
12268176 12268287 28398 28509 - exon coding_transcript
12268176 12268287 28398 28509 - cds coding_transcript
12268288 12269989 28510 30211 - intron coding_transcript
12269990 12270366 30212 30588 - exon coding_transcript
12269990 12270366 30212 30588 - exon coding_transcript
12269990 12270274 30212 30496 - cds coding_transcript
12269990 12270274 30212 30496 - cds coding_transcript
12270275 12270366 30497 30588 - five_prime_utr coding_transcript
12270275 12270366 30497 30588 - five_prime_utr coding_transcript
12270793 12274576 31015 34798 + gene CG10426 coding_transcript
12284056 12289121 44278 49343 - gene CG4328 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG4300[24720]::S000169_fly_pretag_rev
GCGCTAGAAGTAGCTACTGCTGCTGCTGAGGAGTCCACCTGAGCTTGTCActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG4300[24720]::S000169_fly_pretag_fwd
TTGTGCCGTCGTTCATGGAAGAGTGGCTCTTCTACCAAGTGACTTTCGCCgaagtgcataccaatcaggacccgc
fasta Fasta