Clone: 8137439615386962 B11

Tagged gene: Fmrf
Identifier: FBgn0000715

Clone FlyFos021666(pRedFlp-Hgr)(Fmrf[19893]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
5798398 5800993 16585 19180 - gene CG12140 coding_transcript
5793796 5797962 19616 24844 - gene Fmrf coding_transcript
5793796 5797962 19616 24844 - mrna FBtr0088383 coding_transcript
5796644 5797962 19616 21996 - exon coding_transcript
5797688 5797962 19616 19890 - three_prime_utr coding_transcript
5796644 5797687 19891 21996 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19967 20000 - misc_recomb FRT CLC
2358 2429 20008 20079 - cds BLRP CLC
2337 2357 20080 20100 - cds TEV CLC
2313 2336 20101 20124 - cds Precision cut site CLC
2271 2312 20125 20166 - cds V5 CLC
1548 2264 20173 20889 - cds SGFP CLC
1482 1541 20896 20955 - cds 2xTY1 CLC
dmel-5.43-2R
5793907 5796643 21997 24733 - intron coding_transcript
5793796 5793906 24734 24844 - exon coding_transcript
5793796 5793906 24734 24844 - five_prime_utr coding_transcript
5786523 5790788 27852 32117 + gene CG1441 coding_transcript
5779496 5786018 32622 39144 - gene CG30007 coding_transcript
5777403 5778625 40015 41237 - gene CG30008 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Fmrf[19893]::S000169_fly_pretag_fwd
CGACCACCACGCAGGATGGGAGTGTGGAACAGGACCAGTTCTTCGGCCAGgaagtgcataccaatcaggacccgc
 fasta Fasta
Fmrf[19893]::S000169_fly_pretag_rev
TACATTTGTCCATATCTATTTACAAGGAGGCGTCCCGCAGGACTACCTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta