Clone: 8137439615386962 H09

Tagged gene: phl
Identifier: FBgn0003079

Clone FlyFos021552(pRedFlp-Hgr)(phl[18227]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
2238030 2238670 17019 17659 + gene mRpL14 coding_transcript
2234159 2237904 17785 22592 - gene phl coding_transcript
2234159 2237904 17785 22592 - mrna FBtr0070401 coding_transcript
2234159 2237904 17785 22592 - mrna FBtr0111025 coding_transcript
2236885 2237904 17785 19866 - exon coding_transcript
2236885 2237904 17785 19866 - exon coding_transcript
2237465 2237904 17785 18224 - three_prime_utr coding_transcript
2237465 2237904 17785 18224 - three_prime_utr coding_transcript
2236885 2237464 18225 19866 - cds coding_transcript
2236885 2237464 18225 19866 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 18301 18334 - misc_recomb FRT CLC
2358 2429 18342 18413 - cds BLRP CLC
2337 2357 18414 18434 - cds TEV CLC
2313 2336 18435 18458 - cds Precision cut site CLC
2271 2312 18459 18500 - cds V5 CLC
1548 2264 18507 19223 - cds SGFP CLC
1482 1541 19230 19289 - cds 2xTY1 CLC
dmel-5.43-X
2236820 2236884 19867 19931 - intron coding_transcript
2236820 2236884 19867 19931 - intron coding_transcript
2236446 2236819 19932 20305 - exon coding_transcript
2236446 2236819 19932 20305 - cds coding_transcript
2236446 2236819 19932 20305 - exon coding_transcript
2236446 2236819 19932 20305 - cds coding_transcript
2236378 2236445 20306 20373 - intron coding_transcript
2236378 2236445 20306 20373 - intron coding_transcript
2236237 2236377 20374 20514 - exon coding_transcript
2236237 2236377 20374 20514 - cds coding_transcript
2236237 2236377 20374 20514 - exon coding_transcript
2236237 2236377 20374 20514 - cds coding_transcript
2236173 2236236 20515 20578 - intron coding_transcript
2236173 2236236 20515 20578 - intron coding_transcript
2234159 2236172 20579 22592 - exon coding_transcript
2234919 2236172 20579 21832 - cds coding_transcript
2235211 2236172 20579 21540 - exon coding_transcript
2235211 2236172 20579 21540 - cds coding_transcript
2235082 2235210 21541 21669 - intron coding_transcript
2234159 2235081 21670 22592 - exon coding_transcript
2234919 2235081 21670 21832 - cds coding_transcript
2234159 2234918 21833 22592 - five_prime_utr coding_transcript
2234159 2234918 21833 22592 - five_prime_utr coding_transcript
2225526 2228691 28060 31225 + gene Ilp6 coding_transcript
2221161 2222974 33777 35590 - gene CG2854 coding_transcript
2218781 2220250 36501 37970 + gene CG14050 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
phl[18227]::S000169_fly_pretag_fwd
CGCCGGTGAACTTCAACAACTTTCAGTTCTTCGGCAGCGCTGGGAATATCgaagtgcataccaatcaggacccgc
 fasta Fasta
phl[18227]::S000169_fly_pretag_rev
CACGTTGATCACGCAGGATATATGTAAGTACAGGTACAGGTCGCTGTCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta