Clone: 8137439615386962 B08
Tagged gene: Glu-RI
Identifier: FBgn0004619
| Clone | FlyFos021053(pRedFlp-Hgr)(Glu-RI[25718]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation(click to hide details)
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | good | [view] |
Genes in pFlyFos vector(click to hide details)
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-3L | ||||||||
| 6617134 | 6628166 | 14992 | 27086 | + | gene | Glu-RI | coding_transcript | |
| 6617134 | 6628166 | 14992 | 27086 | + | mrna | FBtr0076982 | coding_transcript | |
| 6617134 | 6617796 | 14992 | 15654 | + | exon | coding_transcript | ||
| 6617134 | 6617669 | 14992 | 15527 | + | five_prime_utr | coding_transcript | ||
| 6617670 | 6617796 | 15528 | 15654 | + | cds | coding_transcript | ||
| 6617797 | 6619520 | 15655 | 17378 | + | intron | coding_transcript | ||
| 6619521 | 6619673 | 17379 | 17531 | + | exon | coding_transcript | ||
| 6619521 | 6619673 | 17379 | 17531 | + | cds | coding_transcript | ||
| 6619674 | 6620030 | 17532 | 17888 | + | intron | coding_transcript | ||
| 6620031 | 6620158 | 17889 | 18016 | + | exon | coding_transcript | ||
| 6620031 | 6620158 | 17889 | 18016 | + | cds | coding_transcript | ||
| 6620159 | 6621975 | 18017 | 19833 | + | intron | coding_transcript | ||
| 6621976 | 6622105 | 19834 | 19963 | + | exon | coding_transcript | ||
| 6621976 | 6622105 | 19834 | 19963 | + | cds | coding_transcript | ||
| 6622106 | 6624400 | 19964 | 22258 | + | intron | coding_transcript | ||
| 6624401 | 6624654 | 22259 | 22512 | + | exon | coding_transcript | ||
| 6624401 | 6624654 | 22259 | 22512 | + | cds | coding_transcript | ||
| 6624655 | 6624966 | 22513 | 22824 | + | intron | coding_transcript | ||
| 6624967 | 6625152 | 22825 | 23010 | + | exon | coding_transcript | ||
| 6624967 | 6625152 | 22825 | 23010 | + | cds | coding_transcript | ||
| 6625153 | 6625240 | 23011 | 23098 | + | intron | coding_transcript | ||
| 6625241 | 6625480 | 23099 | 23338 | + | exon | coding_transcript | ||
| 6625241 | 6625480 | 23099 | 23338 | + | cds | coding_transcript | ||
| 6625481 | 6625688 | 23339 | 23546 | + | intron | coding_transcript | ||
| 6625689 | 6626049 | 23547 | 23907 | + | exon | coding_transcript | ||
| 6625689 | 6626049 | 23547 | 23907 | + | cds | coding_transcript | ||
| 6626050 | 6626172 | 23908 | 24030 | + | intron | coding_transcript | ||
| 6626173 | 6626435 | 24031 | 24293 | + | exon | coding_transcript | ||
| 6626173 | 6626435 | 24031 | 24293 | + | cds | coding_transcript | ||
| 6626436 | 6626499 | 24294 | 24357 | + | intron | coding_transcript | ||
| 6626500 | 6626958 | 24358 | 24816 | + | exon | coding_transcript | ||
| 6626500 | 6626958 | 24358 | 24816 | + | cds | coding_transcript | ||
| 6626959 | 6627021 | 24817 | 24879 | + | intron | coding_transcript | ||
| 6627022 | 6627266 | 24880 | 25124 | + | exon | coding_transcript | ||
| 6627022 | 6627266 | 24880 | 25124 | + | cds | coding_transcript | ||
| 6627267 | 6627366 | 25125 | 25224 | + | intron | coding_transcript | ||
| 6627367 | 6627760 | 25225 | 25618 | + | exon | coding_transcript | ||
| 6627367 | 6627760 | 25225 | 25618 | + | cds | coding_transcript | ||
| 6627761 | 6627827 | 25619 | 25685 | + | intron | coding_transcript | ||
| 6627828 | 6628166 | 25686 | 27086 | + | exon | coding_transcript | ||
| 6627828 | 6627863 | 25686 | 26783 | + | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 1482 | 1541 | 25719 | 25778 | + | cds | 2xTY1 | CLC | |
| 1548 | 2264 | 25785 | 26501 | + | cds | SGFP | CLC | |
| 2271 | 2312 | 26508 | 26549 | + | cds | V5 | CLC | |
| 2313 | 2336 | 26550 | 26573 | + | cds | Precision cut site | CLC | |
| 2337 | 2357 | 26574 | 26594 | + | cds | TEV | CLC | |
| 2358 | 2429 | 26595 | 26666 | + | cds | BLRP | CLC | |
| 3813 | 3846 | 26674 | 26707 | + | misc_recomb | FRT | CLC | |
| dmel-5.43-3L | ||||||||
| 6627864 | 6628166 | 26784 | 27086 | + | three_prime_utr | coding_transcript | ||
Sets(click to hide details)
Oligos(click to hide details)
| Name/Sequence | Export |
|---|---|
|
Glu-RI[25718]::S000169_fly_pretag_rev
TGCCTTAATTATTTACAAGTTACTCGTATGGTGAACGAGCGGGTTTCTTActtgtcgtcgtcatccttgtagtca
|
Fasta
|
|
Glu-RI[25718]::S000169_fly_pretag_fwd
ACTTTTTTAACCCCCAGTTGGCCTCGAACGTGCGGTATCAGTACAGCATGgaagtgcataccaatcaggacccgc
|
Fasta
|