Clone: 8137439615386962 E07

Tagged gene: fliI
Identifier: FBgn0000709

Clone FlyFos020949(pRedFlp-Hgr)(fliI[28091]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
21202369 21209988 15251 22870 - gene tty coding_transcript
21210823 21216117 23705 30061 + gene fliI coding_transcript
21210823 21216117 23705 30061 + mrna FBtr0077192 coding_transcript
21210823 21210974 23705 23856 + exon coding_transcript
21210823 21210920 23705 23802 + five_prime_utr coding_transcript
21210921 21210974 23803 23856 + cds coding_transcript
21210975 21211137 23857 24019 + intron coding_transcript
21211138 21214292 24020 27174 + exon coding_transcript
21211138 21214292 24020 27174 + cds coding_transcript
21214293 21214527 27175 27409 + intron coding_transcript
21214528 21214678 27410 27560 + exon coding_transcript
21214528 21214678 27410 27560 + cds coding_transcript
21214679 21214801 27561 27683 + intron coding_transcript
21214802 21216117 27684 30061 + exon coding_transcript
21214802 21215212 27684 29156 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 28092 28151 + cds 2xTY1 CLC
1548 2264 28158 28874 + cds SGFP CLC
2271 2312 28881 28922 + cds V5 CLC
2313 2336 28923 28946 + cds Precision cut site CLC
2337 2357 28947 28967 + cds TEV CLC
2358 2429 28968 29039 + cds BLRP CLC
3813 3846 29047 29080 + misc_recomb FRT CLC
dmel-5.43-X
21215213 21216117 29157 30061 + three_prime_utr coding_transcript
21216375 21217885 30319 31829 + gene dod coding_transcript
21217530 21220866 31474 34810 - gene pen coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
fliI[28091]::S000169_fly_pretag_fwd
GCCGTTTCACCAAGTGCTTCCACGGCTGGAGCGCCTTCAAGGTGTATTTAgaagtgcataccaatcaggacccgc
 fasta Fasta
fliI[28091]::S000169_fly_pretag_rev
GGAAATTTAAAAGAACTACTCGACGATGCTCCTTCAGTTGGCTAAAATTActtgtcgtcgtcatccttgtagtca
 fasta Fasta