Clone: 8137439615386962 E02

Tagged gene: spd-2
Identifier: FBgn0027500

Clone FlyFos020075(pRedFlp-Hgr)(spd-2[26158]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
16569959 16573387 11611 15039 - gene nxf2 coding_transcript
16573498 16574374 15150 16026 - gene Smn coding_transcript
16576083 16577122 17735 18774 + gene Rpn12 coding_transcript
16577224 16579211 18876 20863 + gene CG4169 coding_transcript
16579208 16580199 20860 21851 - gene Syx8 coding_transcript
16580433 16581243 22085 22895 + gene CG32163 coding_transcript
16581575 16583232 23227 24884 + gene l(3)73Ah coding_transcript
16583159 16584150 24811 25802 - gene tra coding_transcript
16584307 16588142 25959 30856 - gene spd-2 coding_transcript
16584307 16588142 25959 30856 - mrna FBtr0075363 coding_transcript
16584307 16587102 25959 29816 - exon coding_transcript
16584307 16584503 25959 26155 - three_prime_utr coding_transcript
16584504 16587102 26156 29816 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 26232 26265 - misc_recomb FRT CLC
2358 2429 26273 26344 - cds BLRP CLC
2337 2357 26345 26365 - cds TEV CLC
2313 2336 26366 26389 - cds Precision cut site CLC
2271 2312 26390 26431 - cds V5 CLC
1548 2264 26438 27154 - cds SGFP CLC
1482 1541 27161 27220 - cds 2xTY1 CLC
dmel-5.43-3L
16587103 16587163 29817 29877 - intron coding_transcript
16587164 16587920 29878 30634 - exon coding_transcript
16587164 16587920 29878 30634 - cds coding_transcript
16587921 16587981 30635 30695 - intron coding_transcript
16587982 16588142 30696 30856 - exon coding_transcript
16587982 16588066 30696 30780 - cds coding_transcript
16588067 16588142 30781 30856 - five_prime_utr coding_transcript
16588519 16593267 31233 35981 + gene CG32165 coding_transcript
16596187 16601254 38901 43968 + gene CG32164 coding_transcript
16601238 16602320 43952 45034 - gene CG4098 coding_transcript
16602497 16603521 45211 46235 - gene Pros26 coding_transcript
16603762 16605297 46476 48011 - gene Su(P) coding_transcript
16605586 16606051 48300 48765 + gene CG4101 coding_transcript
16606318 16608572 49032 51286 - gene Mo25 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
spd-2[26158]::S000169_fly_pretag_fwd
TGGTCTCCATGGAAAACGAGCGCATCAGTGTCCCGATTAGTTTTAAATTTgaagtgcataccaatcaggacccgc
 fasta Fasta
spd-2[26158]::S000169_fly_pretag_rev
CACAAACATAGGGAAAGCAGAATTAGCAGGCACAAATAAAACAAATTTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta