Clone: 3861840049532044 G12

Tagged gene: RecQ5
Identifier: FBgn0027375

Clone FlyFos017845(pRedFlp-Hgr)(RecQ5[42556]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
14621260 14625173 38787 43762 + gene RecQ5 coding_transcript
14621260 14625173 38787 43762 + mrna FBtr0075714 coding_transcript
14621260 14625173 38787 43762 + mrna FBtr0075715 coding_transcript
14621260 14625173 38787 43762 + mrna FBtr0100362 coding_transcript
14624981 14625173 38787 38979 + exon coding_transcript
14624981 14625173 38787 38979 + exon coding_transcript
14624981 14625173 38787 38979 + exon coding_transcript
14625084 14625173 38787 38876 + five_prime_utr coding_transcript
14625084 14625173 38787 38876 + five_prime_utr coding_transcript
14625084 14625173 38787 38876 + five_prime_utr coding_transcript
14624981 14625083 38877 38979 + cds coding_transcript
14624981 14625083 38877 38979 + cds coding_transcript
14624981 14625083 38877 38979 + cds coding_transcript
14624919 14624980 38980 39041 + intron coding_transcript
14624919 14624980 38980 39041 + intron coding_transcript
14624919 14624980 38980 39041 + intron coding_transcript
14622841 14624918 39042 41119 + exon coding_transcript
14623609 14624918 39042 40351 + cds coding_transcript
14623620 14624918 39042 40340 + exon coding_transcript
14623620 14624918 39042 40340 + cds coding_transcript
14623620 14624918 39042 40340 + exon coding_transcript
14623620 14624918 39042 40340 + cds coding_transcript
14623293 14623619 40341 40667 + intron coding_transcript
14623545 14623619 40341 40415 + intron coding_transcript
14622841 14623608 40352 41119 + three_prime_utr coding_transcript
14622841 14623544 40416 41119 + exon coding_transcript
14623540 14623544 40416 40420 + cds coding_transcript
14622841 14623539 40421 41119 + three_prime_utr coding_transcript
14622841 14623292 40668 41119 + exon coding_transcript
14622841 14623292 40668 41119 + cds coding_transcript
14622782 14622840 41120 41178 + intron coding_transcript
14622782 14622840 41120 41178 + intron coding_transcript
14622782 14622840 41120 41178 + intron coding_transcript
14621563 14622781 41179 42397 + exon coding_transcript
14621563 14622781 41179 42397 + three_prime_utr coding_transcript
14621563 14622781 41179 42397 + exon coding_transcript
14621563 14622781 41179 42397 + cds coding_transcript
14621563 14622781 41179 42397 + exon coding_transcript
14621563 14622781 41179 42397 + three_prime_utr coding_transcript
14621505 14621562 42398 42455 + intron coding_transcript
14621505 14621562 42398 42455 + intron coding_transcript
14621505 14621562 42398 42455 + intron coding_transcript
14621260 14621504 42456 43762 + exon coding_transcript
14621260 14621504 42456 43762 + three_prime_utr coding_transcript
14621260 14621504 42456 43762 + exon coding_transcript
14621260 14621504 42456 43762 + exon coding_transcript
14621260 14621504 42456 43762 + three_prime_utr coding_transcript
14621401 14621504 42456 43621 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 42557 42616 + cds 2xTY1 CLC
1548 2264 42623 43339 + cds SGFP CLC
2271 2312 43346 43387 + cds V5 CLC
2313 2336 43388 43411 + cds Precision cut site CLC
2337 2357 43412 43432 + cds TEV CLC
2358 2429 43433 43504 + cds BLRP CLC
3813 3846 43512 43545 + misc_recomb FRT CLC
dmel-5.43-3L
14621260 14621400 43622 43762 + three_prime_utr coding_transcript
14615910 14621274 43748 49112 - gene CG9628 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
RecQ5[42556]::S000169_fly_pretag_rev
ACGAGGCGAAACAATATGTCGTGATAAAATCATTAGGAGATTTTTAGTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
RecQ5[42556]::S000169_fly_pretag_fwd
AGATGATAAACAACGATCAGGACATTGAAAAATATTTCATAAAATCCAAGgaagtgcataccaatcaggacccgc
 fasta Fasta