Clone: 3861840049532044 C01

Tagged gene: tobi
Identifier: FBgn0261575

Clone FlyFos016263(pRedFlp-Hgr)(tobi[26535]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
21184007 21185756 11360 13109 - gene CG11913 coding_transcript
21170483 21172634 24482 27695 + gene tobi coding_transcript
21170483 21172634 24482 27695 + mrna FBtr0084847 coding_transcript
21170835 21172634 24482 26281 + exon coding_transcript
21172609 21172634 24482 24507 + five_prime_utr coding_transcript
21170835 21172608 24508 26281 + cds coding_transcript
21170778 21170834 26282 26338 + intron coding_transcript
21170483 21170777 26339 27695 + exon coding_transcript
21170578 21170777 26339 27600 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 26536 26595 + cds 2xTY1 CLC
1548 2264 26602 27318 + cds SGFP CLC
2271 2312 27325 27366 + cds V5 CLC
2313 2336 27367 27390 + cds Precision cut site CLC
2337 2357 27391 27411 + cds TEV CLC
2358 2429 27412 27483 + cds BLRP CLC
3813 3846 27491 27524 + misc_recomb FRT CLC
dmel-5.43-3R
21170483 21170577 27601 27695 + three_prime_utr coding_transcript
21165974 21170560 27618 32204 - gene rha coding_transcript
21161033 21165810 32368 37145 - gene CG11902 coding_transcript
21157085 21161006 37172 41093 + gene CG10669 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
tobi[26535]::S000169_fly_pretag_rev
TTAAATATACACGCTAGGAAGTTTAGATATATAAAACGGTTTCCGCTCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
tobi[26535]::S000169_fly_pretag_fwd
CTCCCTTGGATACCCTACCCTACTTTTTGCGCGTGGGATTCACACTGGAGgaagtgcataccaatcaggacccgc
 fasta Fasta