Clone: 6656113284557374 E05

Tagged gene: Sos
Identifier: FBgn0001965

Clone FlyFos015364(pRedFlp-Hgr)(Sos[32330]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
13803628 13809994 16583 22949 + gene CAH1 coding_transcript
13808360 13811472 21315 24427 - gene adat coding_transcript
13811526 13812682 24481 25637 + gene CG16865 coding_transcript
13812700 13813234 25655 26189 - gene CG16888 coding_transcript
13813828 13819826 26783 33843 + gene Sos coding_transcript
13813828 13819826 26783 33843 + mrna FBtr0080541 coding_transcript
13813828 13815305 26783 28260 + exon coding_transcript
13813828 13814213 26783 27168 + five_prime_utr coding_transcript
13814214 13815305 27169 28260 + cds coding_transcript
13815306 13815358 28261 28313 + intron coding_transcript
13815359 13815716 28314 28671 + exon coding_transcript
13815359 13815716 28314 28671 + cds coding_transcript
13815717 13815778 28672 28733 + intron coding_transcript
13815779 13816701 28734 29656 + exon coding_transcript
13815779 13816701 28734 29656 + cds coding_transcript
13816702 13816765 29657 29720 + intron coding_transcript
13816766 13817614 29721 30569 + exon coding_transcript
13816766 13817614 29721 30569 + cds coding_transcript
13817615 13817676 30570 30631 + intron coding_transcript
13817677 13818567 30632 31522 + exon coding_transcript
13817677 13818567 30632 31522 + cds coding_transcript
13818568 13818635 31523 31590 + intron coding_transcript
13818636 13819269 31591 32224 + exon coding_transcript
13818636 13819269 31591 32224 + cds coding_transcript
13819270 13819334 32225 32289 + intron coding_transcript
13819335 13819826 32290 33843 + exon coding_transcript
13819335 13819378 32290 33395 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 32331 32390 + cds 2xTY1 CLC
1548 2264 32397 33113 + cds SGFP CLC
2271 2312 33120 33161 + cds V5 CLC
2313 2336 33162 33185 + cds Precision cut site CLC
2337 2357 33186 33206 + cds TEV CLC
2358 2429 33207 33278 + cds BLRP CLC
3813 3846 33286 33319 + misc_recomb FRT CLC
dmel-5.43-2L
13819379 13819826 33396 33843 + three_prime_utr coding_transcript
13821203 13823979 35220 37996 + gene b coding_transcript
13823980 13827793 37997 41810 - gene tam coding_transcript
13827941 13829748 41958 43765 - gene Sop2 coding_transcript
13829857 13831576 43874 45593 + gene Orc5 coding_transcript
13831482 13833167 45499 47184 - gene CG33649 coding_transcript
13831482 13833167 45499 47184 - gene DNApol-gamma35 coding_transcript
13833339 13834306 47356 48323 + gene RpII33 coding_transcript
13834250 13835056 48267 49073 - gene mRpS23 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Sos[32330]::S000169_fly_pretag_fwd
CGTTTGCAGATAAAGGTACGATGTTTCTTTACCCAAGTACAAACGAAGAAgaagtgcataccaatcaggacccgc
 fasta Fasta
Sos[32330]::S000169_fly_pretag_rev
ATCATACTCGTAGTTTTCGTCCTTATCACATCGCCAGGCAATTTAAATTActtgtcgtcgtcatccttgtagtca
 fasta Fasta