Clone: 6656113284557374 C04

Tagged gene: pygo
Identifier: FBgn0043900

Clone FlyFos015262(pRedFlp-Hgr)(pygo[31470]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad
Whole tag sequencing incomplete download [show/hide details]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
27420509 27421967 12120 13578 + gene RpL6 coding_transcript
27418466 27420449 13638 15621 - gene CG1774 coding_transcript
27416303 27418041 16046 17784 - gene CG1638 coding_transcript
27414390 27416111 17976 19697 - gene CG1635 coding_transcript
27406394 27413861 20226 27693 - gene rod coding_transcript
27401557 27406412 27675 33592 + gene pygo coding_transcript
27401557 27406412 27675 33592 + mrna FBtr0085806 coding_transcript
27405675 27406412 27675 28412 + exon coding_transcript
27405675 27406412 27675 28412 + five_prime_utr coding_transcript
27405608 27405674 28413 28479 + intron coding_transcript
27403026 27405607 28480 31061 + exon coding_transcript
27405353 27405607 28480 28734 + five_prime_utr coding_transcript
27403026 27405352 28735 31061 + cds coding_transcript
27402735 27403025 31062 31352 + intron coding_transcript
27401557 27402734 31353 33592 + exon coding_transcript
27402614 27402734 31353 32535 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 31471 31530 + cds 2xTY1 CLC
1548 2264 31537 32253 + cds SGFP CLC
2271 2312 32260 32301 + cds V5 CLC
2313 2336 32302 32325 + cds Precision cut site CLC
2337 2357 32326 32346 + cds TEV CLC
2358 2429 32347 32418 + cds BLRP CLC
3813 3846 32426 32459 + misc_recomb FRT CLC
dmel-5.43-3R
27401557 27402613 32536 33592 + three_prime_utr coding_transcript
27397865 27401429 33720 37284 - gene gammaCop coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
pygo[31470]::S000169_fly_pretag_rev
GCTGGTGGTTGATCGATGTGTTTTGCTGATTTCAAATCCGATCCGCTTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
pygo[31470]::S000169_fly_pretag_fwd
GCGACAAGTGCGTGTCTTCCAAGCATATTCCCATGGTCAAGTTCAAGTGTgaagtgcataccaatcaggacccgc
 fasta Fasta