Clone: 16945536021279262 C12

Tagged gene: sage
Identifier: FBgn0037672

Clone FlyFos031933(pRedFlp-Hgr)(sage[22689]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
5092754 5095958 15331 18535 - gene Vps16A coding_transcript
5089539 5092670 18619 21750 + gene Aats-trp coding_transcript
5088383 5089464 21825 23968 + gene sage coding_transcript
5088383 5089464 21825 23968 + mrna FBtr0081982 coding_transcript
5088797 5089464 21825 22492 + exon coding_transcript
5088797 5089464 21825 22492 + cds coding_transcript
5088383 5089278 22011 23968 + mrna FBtr0081983 coding_transcript
5088797 5089278 22011 22492 + exon coding_transcript
5089195 5089278 22011 22094 + five_prime_utr coding_transcript
5088797 5089194 22095 22492 + cds coding_transcript
5088736 5088796 22493 22553 + intron coding_transcript
5088736 5088796 22493 22553 + intron coding_transcript
5088383 5088735 22554 23968 + exon coding_transcript
5088383 5088735 22554 23968 + exon coding_transcript
5088597 5088735 22554 23754 + cds coding_transcript
5088597 5088735 22554 23754 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 22690 22749 + cds 2xTY1 CLC
1548 2264 22756 23472 + cds SGFP CLC
2271 2312 23479 23520 + cds V5 CLC
2313 2336 23521 23544 + cds Precision cut site CLC
2337 2357 23545 23565 + cds TEV CLC
2358 2429 23566 23637 + cds BLRP CLC
3813 3846 23645 23678 + misc_recomb FRT CLC
dmel-5.43-3R
5088383 5088596 23755 23968 + three_prime_utr coding_transcript
5088383 5088596 23755 23968 + three_prime_utr coding_transcript
5086363 5088315 24036 25988 - gene VhaM8.9 coding_transcript
5084691 5086122 26229 27660 - gene CG8436 coding_transcript
5083449 5084223 28128 28902 + gene CG9740 coding_transcript
5082411 5083438 28913 29940 + gene CG16736 coding_transcript
5080774 5082453 29898 31577 - gene CG16734 coding_transcript
5077575 5078687 33664 34776 - gene CG16733 coding_transcript
5073173 5077160 35191 39178 - gene CG8420 coding_transcript
5066479 5071540 40811 45872 + gene ird1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
sage[22689]::S000169_fly_pretag_fwd
GCGGCGGAAGTAGTTCACCTTATGATAATGGCAACGATCATTGGGGAGCGgaagtgcataccaatcaggacccgc
 fasta Fasta
sage[22689]::S000169_fly_pretag_rev
TAAATTGAATATTTAAATGAATTCCCTTTGATATTCGTTAATTCAAGTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta