Clone: 6248143701161187 B07

Tagged gene: knk
Identifier: FBgn0001321

Clone FlyFos018513(pRedFlp-Hgr)(knk[27968]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
5938268 5945142 17988 24862 + gene CG3940 coding_transcript
5946121 5947122 25841 26842 - gene CG6208 coding_transcript
5947523 5948042 27243 27762 + gene CG31477 coding_transcript
5948087 5951383 27807 32165 - gene knk coding_transcript
5948087 5951383 27807 32165 - mrna FBtr0082194 coding_transcript
5948087 5948873 27807 29655 - exon coding_transcript
5948087 5948245 27807 27965 - three_prime_utr coding_transcript
5948246 5948873 27966 29655 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 28042 28075 - misc_recomb FRT CLC
2358 2429 28083 28154 - cds BLRP CLC
2337 2357 28155 28175 - cds TEV CLC
2313 2336 28176 28199 - cds Precision cut site CLC
2271 2312 28200 28241 - cds V5 CLC
1548 2264 28248 28964 - cds SGFP CLC
1482 1541 28971 29030 - cds 2xTY1 CLC
dmel-5.43-3R
5948874 5948927 29656 29709 - intron coding_transcript
5948928 5949153 29710 29935 - exon coding_transcript
5948928 5949153 29710 29935 - cds coding_transcript
5949154 5949215 29936 29997 - intron coding_transcript
5949216 5949769 29998 30551 - exon coding_transcript
5949216 5949769 29998 30551 - cds coding_transcript
5949770 5949841 30552 30623 - intron coding_transcript
5949842 5950189 30624 30971 - exon coding_transcript
5949842 5950189 30624 30971 - cds coding_transcript
5950190 5950263 30972 31045 - intron coding_transcript
5950264 5950486 31046 31268 - exon coding_transcript
5950264 5950486 31046 31268 - cds coding_transcript
5950487 5950850 31269 31632 - intron coding_transcript
5950851 5951383 31633 32165 - exon coding_transcript
5950851 5950941 31633 31723 - cds coding_transcript
5950942 5951383 31724 32165 - five_prime_utr coding_transcript
5952523 5965492 33305 46274 - gene tws coding_transcript
5968809 5969111 49591 49893 + gene CG42759 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
knk[27968]::S000169_fly_pretag_rev
ATTTAGACTAAGACACTAAATAGGAGAATCTAAGAATGAGAAAACAGTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
knk[27968]::S000169_fly_pretag_fwd
TATCCTGGTCCTTGTGCATCATCCTGCTACCGTGGCTCGTGCTCCAGAACgaagtgcataccaatcaggacccgc
 fasta Fasta