Clone: 5906201510157786 H12

Tagged gene: CG33271
Identifier: FBgn0053271

Clone FlyFos031538(pRedFlp-Hgr)(CG33271[20713]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
11565897 11567763 16339 18205 - gene CG33490 coding_transcript
11567864 11570144 18306 20586 - gene CG33489 coding_transcript
11570106 11570595 20548 22099 - gene CG33271 coding_transcript
11570106 11570595 20548 22099 - mrna FBtr0076150 coding_transcript
11570106 11570595 20548 22099 - exon coding_transcript
11570106 11570268 20548 20710 - three_prime_utr coding_transcript
11570269 11570535 20711 22039 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 20787 20820 - misc_recomb FRT CLC
2358 2429 20828 20899 - cds BLRP CLC
2337 2357 20900 20920 - cds TEV CLC
2313 2336 20921 20944 - cds Precision cut site CLC
2271 2312 20945 20986 - cds V5 CLC
1548 2264 20993 21709 - cds SGFP CLC
1482 1541 21716 21775 - cds 2xTY1 CLC
dmel-5.43-3L
11570536 11570595 22040 22099 - five_prime_utr coding_transcript
11571336 11571723 22840 23227 - gene CG33270 coding_transcript
11572843 11573050 24347 24554 - gene CG43390 coding_transcript
11573929 11574119 25433 25623 - gene CG43391 coding_transcript
11574515 11575297 26019 26801 - gene CG33269 coding_transcript
11575501 11577164 27005 28668 - gene CG32086 coding_transcript
11577222 11580867 28726 32371 - gene rt coding_transcript
11582795 11583079 34299 34583 + gene CG7377 coding_transcript
11584038 11584322 35542 35826 - gene CG33268 coding_transcript

Sets

Oligos

Name/Sequence Export
CG33271[20713]::S000169_fly_pretag_rev
TAACTGTTAAATGTTTTATTTTCTTCAAACTTGTTGATCTTAAGCCCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG33271[20713]::S000169_fly_pretag_fwd
ACCCAAAGAGATGCCACAAGACCATCGTGATTGTGACCCACAGAAAGAACgaagtgcataccaatcaggacccgc
fasta Fasta