Clone: 5906201510157786 E11

Tagged gene: EndoGI
Identifier: FBgn0028515

Clone FlyFos031527(pRedFlp-Hgr)(EndoGI[21515]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
16262123 16263483 17292 18652 - gene CG4935 coding_transcript
16259416 16261875 18900 21359 + gene twe coding_transcript
16257993 16259433 21342 23844 - gene EndoGI coding_transcript
16257993 16259433 21342 23844 - mrna FBtr0080824 coding_transcript
16258249 16259433 21342 23588 - exon coding_transcript
16259263 16259433 21342 21512 - three_prime_utr coding_transcript
16258249 16259262 21513 23588 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 21589 21622 - misc_recomb FRT CLC
2358 2429 21630 21701 - cds BLRP CLC
2337 2357 21702 21722 - cds TEV CLC
2313 2336 21723 21746 - cds Precision cut site CLC
2271 2312 21747 21788 - cds V5 CLC
1548 2264 21795 22511 - cds SGFP CLC
1482 1541 22518 22577 - cds 2xTY1 CLC
dmel-5.43-2L
16258180 16258248 23589 23657 - intron coding_transcript
16257993 16258179 23658 23844 - exon coding_transcript
16258114 16258179 23658 23723 - cds coding_transcript
16257993 16258113 23724 23844 - five_prime_utr coding_transcript
16245826 16257603 24234 36011 + gene PRL-1 coding_transcript
16242081 16245459 36378 39756 - gene CG42818 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
EndoGI[21515]::S000169_fly_pretag_fwd
ACGAAAAGGCTGGTTGCAAGGCTCCCTTTGAAGGACCTGTAAATGATGATgaagtgcataccaatcaggacccgc
 fasta Fasta
EndoGI[21515]::S000169_fly_pretag_rev
GTTTTAGTACATAAGGAAACAATCCTTCAGTCATGAAAGATGCACTACTActtgtcgtcgtcatccttgtagtca
 fasta Fasta