Clone: 5906201510157786 F04

Tagged gene: Elf
Identifier: FBgn0020443

Clone FlyFos031433(pRedFlp-Hgr)(Elf[20594]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
12446224 12448113 12043 13932 + gene CG5421 coding_transcript
12441475 12445922 14234 18681 + gene Oatp33Ea coding_transcript
12434432 12440605 19551 26786 - gene Elf coding_transcript
12434432 12440605 19551 26786 - mrna FBtr0080369 coding_transcript
12439521 12440605 19551 21697 - exon coding_transcript
12439565 12440605 19551 20591 - three_prime_utr coding_transcript
12439521 12439564 20592 21697 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 20668 20701 - misc_recomb FRT CLC
2358 2429 20709 20780 - cds BLRP CLC
2337 2357 20781 20801 - cds TEV CLC
2313 2336 20802 20825 - cds Precision cut site CLC
2271 2312 20826 20867 - cds V5 CLC
1548 2264 20874 21590 - cds SGFP CLC
1482 1541 21597 21656 - cds 2xTY1 CLC
dmel-5.43-2L
12439465 12439520 21698 21753 - intron coding_transcript
12439296 12439464 21754 21922 - exon coding_transcript
12439296 12439464 21754 21922 - cds coding_transcript
12439231 12439295 21923 21987 - intron coding_transcript
12439054 12439230 21988 22164 - exon coding_transcript
12439054 12439230 21988 22164 - cds coding_transcript
12438989 12439053 22165 22229 - intron coding_transcript
12438902 12438988 22230 22316 - exon coding_transcript
12438902 12438988 22230 22316 - cds coding_transcript
12438842 12438901 22317 22376 - intron coding_transcript
12438072 12438841 22377 23146 - exon coding_transcript
12438072 12438841 22377 23146 - cds coding_transcript
12437999 12438071 23147 23219 - intron coding_transcript
12437813 12437998 23220 23405 - exon coding_transcript
12437813 12437998 23220 23405 - cds coding_transcript
12435424 12437812 23406 25794 - intron coding_transcript
12434432 12435423 25795 26786 - exon coding_transcript
12434997 12435423 25795 26221 - cds coding_transcript
12434432 12434996 26222 26786 - five_prime_utr coding_transcript
12431075 12434665 26553 30143 + gene SC35 coding_transcript
12434559 12434665 26553 26659 + exon coding_transcript
12434559 12434665 26553 26659 + five_prime_utr coding_transcript
12429827 12430892 30326 31391 + gene CG5435 coding_transcript
12428032 12430021 31197 33186 - gene CG6388 coding_transcript
12426899 12427663 33555 34319 + gene CG5446 coding_transcript
12424904 12426440 34778 36314 - gene CG6405 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Elf[20594]::S000169_fly_pretag_rev
CAAAAATCGCAATGAAAGGCGGGCGCTCGAATTTAGCTAGGCTCAATTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Elf[20594]::S000169_fly_pretag_fwd
ATCTTTCAGACAAAACCATTGCCATTGGCAAGGTGCTGAAGGTGGTCGAAgaagtgcataccaatcaggacccgc
fasta Fasta