Clone: 5906201510157786 H01

Tagged gene: Rpb5
Identifier: FBgn0033571

Clone FlyFos031401(pRedFlp-Hgr)(Rpb5[18637]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
6780528 6781865 13326 14663 - gene cag coding_transcript
6782016 6784727 14814 17525 + gene CG12942 coding_transcript
6784641 6785388 17439 18186 - gene CG7712 coding_transcript
6785747 6786563 18545 20423 - gene Rpb5 coding_transcript
6785747 6786563 18545 20423 - mrna FBtr0088237 coding_transcript
6785747 6786563 18545 20423 - exon coding_transcript
6785747 6785836 18545 18634 - three_prime_utr coding_transcript
6785837 6786469 18635 20329 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 18711 18744 - misc_recomb FRT CLC
2358 2429 18752 18823 - cds BLRP CLC
2337 2357 18824 18844 - cds TEV CLC
2313 2336 18845 18868 - cds Precision cut site CLC
2271 2312 18869 18910 - cds V5 CLC
1548 2264 18917 19633 - cds SGFP CLC
1482 1541 19640 19699 - cds 2xTY1 CLC
dmel-5.43-2R
6786470 6786563 20330 20423 - five_prime_utr coding_transcript
6791075 6793068 24935 26928 + gene CG12943 coding_transcript
6797428 6797990 31288 31850 + gene Obp47a coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Rpb5[18637]::S000169_fly_pretag_rev
CTTTTCCTTAAATCATAGGTTTCTTAAAATAATTCATAAGTCCAGGATCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
Rpb5[18637]::S000169_fly_pretag_fwd
TCCGTTCCTCTGAGACGGCGGGACGGTACATATCCTATCGATTGGTGTGCgaagtgcataccaatcaggacccgc
 fasta Fasta