Clone: 6581804864395902 G03

Tagged gene: Pkg21D
Identifier: FBgn0000442

Clone FlyFos030346(pRedFlp-Hgr)(Pkg21D[41526]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
816023 817032 10757 11766 - gene pex12 coding_transcript
813314 815951 11838 14475 + gene Saf6 coding_transcript
811609 813380 14409 16180 - gene KH1 coding_transcript
810586 811531 16258 17203 - gene Nnf1b coding_transcript
786153 790795 36994 42698 + gene Pkg21D coding_transcript
786153 790795 36994 42698 + mrna FBtr0078042 coding_transcript
790489 790795 36994 37300 + exon coding_transcript
790630 790795 36994 37159 + five_prime_utr coding_transcript
790489 790629 37160 37300 + cds coding_transcript
788613 790488 37301 39176 + intron coding_transcript
788242 788612 39177 39547 + exon coding_transcript
788242 788612 39177 39547 + cds coding_transcript
788179 788241 39548 39610 + intron coding_transcript
786733 788178 39611 41056 + exon coding_transcript
786733 788178 39611 41056 + cds coding_transcript
786665 786732 41057 41124 + intron coding_transcript
786479 786664 41125 41310 + exon coding_transcript
786479 786664 41125 41310 + cds coding_transcript
786423 786478 41311 41366 + intron coding_transcript
786153 786422 41367 42698 + exon coding_transcript
786260 786422 41367 42591 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 41527 41586 + cds 2xTY1 CLC
1548 2264 41593 42309 + cds SGFP CLC
2271 2312 42316 42357 + cds V5 CLC
2313 2336 42358 42381 + cds Precision cut site CLC
2337 2357 42382 42402 + cds TEV CLC
2358 2429 42403 42474 + cds BLRP CLC
3813 3846 42482 42515 + misc_recomb FRT CLC
dmel-5.43-2L
786153 786259 42592 42698 + three_prime_utr coding_transcript
784336 786161 42690 44515 - gene CG3544 coding_transcript
780482 782885 45966 48369 - gene Gr21a coding_transcript
779201 779655 49196 49650 - gene CG13947 coding_transcript
776536 776892 51959 52315 - gene CG13946 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Pkg21D[41526]::S000169_fly_pretag_rev
ACTAAAGAGTGATCAATTACTAAGTCAAGGAGGTCGACGGAATGGGCCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
Pkg21D[41526]::S000169_fly_pretag_fwd
GCGATTTGAACGAGCCACCGGACGAGCTCTCCGGCTGGGATGCGGATTTCgaagtgcataccaatcaggacccgc
 fasta Fasta