Clone: 6475152880377884 D02

Tagged gene: CG31860
Identifier: FBgn0051860

Clone FlyFos029759(pRedFlp-Hgr)(CG31860[25435]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
12161795 12162607 20602 21414 + gene CG31861 coding_transcript
12164241 12166723 23048 26592 + gene CG31860 coding_transcript
12164241 12166723 23048 26592 + mrna FBtr0302984 coding_transcript
12164241 12166710 23048 26579 + mrna FBtr0302985 coding_transcript
12164241 12165592 23048 24399 + exon coding_transcript
12164241 12165585 23048 24392 + exon coding_transcript
12164241 12164318 23048 23125 + five_prime_utr coding_transcript
12164241 12164318 23048 23125 + five_prime_utr coding_transcript
12164319 12165592 23126 24399 + cds coding_transcript
12164319 12165585 23126 24392 + cds coding_transcript
12165586 12165792 24393 24599 + intron coding_transcript
12165593 12165792 24400 24599 + intron coding_transcript
12165793 12166723 24600 26592 + exon coding_transcript
12165793 12166710 24600 26579 + exon coding_transcript
12165793 12166631 24600 26500 + cds coding_transcript
12165793 12165829 24600 24636 + cds coding_transcript
12165830 12166710 24637 26579 + three_prime_utr coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 25436 25495 + cds 2xTY1 CLC
1548 2264 25502 26218 + cds SGFP CLC
2271 2312 26225 26266 + cds V5 CLC
2313 2336 26267 26290 + cds Precision cut site CLC
2337 2357 26291 26311 + cds TEV CLC
2358 2429 26312 26383 + cds BLRP CLC
3813 3846 26391 26424 + misc_recomb FRT CLC
dmel-5.43-2L
12166632 12166723 26501 26592 + three_prime_utr coding_transcript
12170586 12171232 30455 31101 + gene CG17217 coding_transcript
12171204 12172569 31073 32438 - gene CG6583 coding_transcript
12172984 12174091 32853 33960 + gene crok coding_transcript
12175448 12177886 35317 37755 - gene atilla coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG31860[25435]::S000169_fly_pretag_fwd
GCAAAAGAGGATCTGATGTGGGAAAAATTAAGAATCACTCGGGATCTGAAgaagtgcataccaatcaggacccgc
fasta Fasta
CG31860[25435]::S000169_fly_pretag_rev
TTCATTTAAAAAGGGCATGTAAATGTAACATGATCAAGTTATTAGATTCActtgtcgtcgtcatccttgtagtca
fasta Fasta