Clone: 6983399659459094 F07

Tagged gene: PH4alphaSG1
Identifier: FBgn0051014

Clone FlyFos029653(pRedFlp-Hgr)(PH4alphaSG1[35985]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
26342774 26352761 12668 22655 + gene jdp coding_transcript
26340546 26342641 22788 24883 - gene CG9698 coding_transcript
26338044 26340077 25352 27385 + gene CG31524 coding_transcript
26335805 26337936 27493 29624 + gene PH4alphaNE2 coding_transcript
26333745 26335868 29561 31684 - gene CG34041 coding_transcript
26331709 26333699 31730 33720 - gene CG15539 coding_transcript
26329387 26331371 34058 37104 + gene PH4alphaSG1 coding_transcript
26329387 26331371 34058 37104 + mrna FBtr0085686 coding_transcript
26331183 26331371 34058 34246 + exon coding_transcript
26331353 26331371 34058 34076 + five_prime_utr coding_transcript
26331183 26331352 34077 34246 + cds coding_transcript
26331122 26331182 34247 34307 + intron coding_transcript
26330443 26331121 34308 34986 + exon coding_transcript
26330443 26331121 34308 34986 + cds coding_transcript
26330386 26330442 34987 35043 + intron coding_transcript
26329936 26330385 35044 35493 + exon coding_transcript
26329936 26330385 35044 35493 + cds coding_transcript
26329877 26329935 35494 35552 + intron coding_transcript
26329817 26329876 35553 35612 + exon coding_transcript
26329817 26329876 35553 35612 + cds coding_transcript
26329759 26329816 35613 35670 + intron coding_transcript
26329593 26329758 35671 35836 + exon coding_transcript
26329593 26329758 35671 35836 + cds coding_transcript
26329539 26329592 35837 35890 + intron coding_transcript
26329387 26329538 35891 37104 + exon coding_transcript
26329441 26329538 35891 37050 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 35986 36045 + cds 2xTY1 CLC
1548 2264 36052 36768 + cds SGFP CLC
2271 2312 36775 36816 + cds V5 CLC
2313 2336 36817 36840 + cds Precision cut site CLC
2337 2357 36841 36861 + cds TEV CLC
2358 2429 36862 36933 + cds BLRP CLC
3813 3846 36941 36974 + misc_recomb FRT CLC
dmel-5.43-3R
26329387 26329440 37051 37104 + three_prime_utr coding_transcript
26326924 26329082 37409 39567 + gene CG31371 coding_transcript
26322473 26326048 40443 44018 + gene PH4alphaNE1 coding_transcript
26317535 26321875 44616 48956 + gene PH4alphaMP coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
PH4alphaSG1[35985]::S000169_fly_pretag_rev
TACAAGTATTTATTTGAAAGGATTACATTATAATTTTACTGCTGTTTTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
PH4alphaSG1[35985]::S000169_fly_pretag_fwd
CTTGTGGCTTGAGCAGTGATGAGGGAAAGTTTTTGCACTTGAAGGACATAgaagtgcataccaatcaggacccgc
 fasta Fasta