Clone: 13996837788417626 H12

Tagged gene: CG5577
Identifier: FBgn0036759

Clone FlyFos029558(pRedFlp-Hgr)(CG5577[19297]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
17826377 17826694 12752 13069 - gene CG32188 coding_transcript
17827844 17828274 14219 14649 - gene CG32189 coding_transcript
17832882 17834504 19257 21941 - gene CG5577 coding_transcript
17832882 17834504 19257 21941 - mrna FBtr0075162 coding_transcript
17832882 17833082 19257 20519 - exon coding_transcript
17832882 17832919 19257 19294 - three_prime_utr coding_transcript
17832920 17833082 19295 20519 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19371 19404 - misc_recomb FRT CLC
2358 2429 19412 19483 - cds BLRP CLC
2337 2357 19484 19504 - cds TEV CLC
2313 2336 19505 19528 - cds Precision cut site CLC
2271 2312 19529 19570 - cds V5 CLC
1548 2264 19577 20293 - cds SGFP CLC
1482 1541 20300 20359 - cds 2xTY1 CLC
dmel-5.43-3L
17833083 17833135 20520 20572 - intron coding_transcript
17833136 17833823 20573 21260 - exon coding_transcript
17833136 17833823 20573 21260 - cds coding_transcript
17833824 17834386 21261 21823 - intron coding_transcript
17834387 17834504 21824 21941 - exon coding_transcript
17834387 17834483 21824 21920 - cds coding_transcript
17834484 17834504 21921 21941 - five_prime_utr coding_transcript
17834635 17836642 22072 24079 - gene CG5567 coding_transcript
17836906 17839600 24343 27037 - gene MED19 coding_transcript
17840016 17842483 27453 29920 + gene CG7430 coding_transcript
17842795 17845554 30232 32991 + gene CG7441 coding_transcript
17845724 17849864 33161 37301 - gene CG5535 coding_transcript
17851574 17853582 39011 41019 + gene CG34002 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG5577[19297]::S000169_fly_pretag_fwd
CCCAGCCAGACTTTTATCTGCCCCGTCTGGGCGATCTGCTGAACATTTTGgaagtgcataccaatcaggacccgc
fasta Fasta
CG5577[19297]::S000169_fly_pretag_rev
GTTGATAACGAGTAGATTGCTGCGCGAGAGTCCCATGGATTTATGGCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta