Clone: 13996837788417626 D11

Tagged gene: CG5521
Identifier: FBgn0039466

Clone FlyFos029533(pRedFlp-Hgr)(CG5521[31005]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
22787446 22790547 16187 19288 - gene Tsp97E coding_transcript
22790866 22792194 19607 20935 - gene Gr97a coding_transcript
22792652 22802267 21393 32070 + gene CG5521 coding_transcript
22792652 22802267 21393 32070 + mrna FBtr0085069 coding_transcript
22792652 22802267 21393 32070 + mrna FBtr0301134 coding_transcript
22792652 22793271 21393 22012 + exon coding_transcript
22792652 22793271 21393 22012 + exon coding_transcript
22792652 22793165 21393 21906 + five_prime_utr coding_transcript
22792652 22793165 21393 21906 + five_prime_utr coding_transcript
22793166 22793271 21907 22012 + cds coding_transcript
22793166 22793271 21907 22012 + cds coding_transcript
22793272 22795406 22013 24147 + intron coding_transcript
22793272 22795406 22013 24147 + intron coding_transcript
22795407 22795517 24148 24258 + exon coding_transcript
22795407 22795517 24148 24258 + cds coding_transcript
22795407 22795517 24148 24258 + exon coding_transcript
22795407 22795517 24148 24258 + cds coding_transcript
22795518 22796373 24259 25114 + intron coding_transcript
22795518 22796048 24259 24789 + intron coding_transcript
22796049 22796066 24790 24807 + exon coding_transcript
22796049 22796066 24790 24807 + cds coding_transcript
22796067 22796373 24808 25114 + intron coding_transcript
22796374 22796545 25115 25286 + exon coding_transcript
22796374 22796545 25115 25286 + cds coding_transcript
22796374 22796545 25115 25286 + exon coding_transcript
22796374 22796545 25115 25286 + cds coding_transcript
22796546 22796604 25287 25345 + intron coding_transcript
22796546 22796604 25287 25345 + intron coding_transcript
22796605 22797571 25346 26312 + exon coding_transcript
22796605 22797571 25346 26312 + cds coding_transcript
22796605 22797571 25346 26312 + exon coding_transcript
22796605 22797571 25346 26312 + cds coding_transcript
22797572 22797631 26313 26372 + intron coding_transcript
22797572 22797631 26313 26372 + intron coding_transcript
22797632 22801698 26373 30439 + exon coding_transcript
22797632 22801698 26373 30439 + cds coding_transcript
22797632 22801698 26373 30439 + exon coding_transcript
22797632 22801698 26373 30439 + cds coding_transcript
22801699 22801754 30440 30495 + intron coding_transcript
22801699 22801754 30440 30495 + intron coding_transcript
22801755 22801951 30496 30692 + exon coding_transcript
22801755 22801951 30496 30692 + cds coding_transcript
22801755 22801951 30496 30692 + exon coding_transcript
22801755 22801951 30496 30692 + cds coding_transcript
22801952 22802010 30693 30751 + intron coding_transcript
22801952 22802010 30693 30751 + intron coding_transcript
22802011 22802267 30752 32070 + exon coding_transcript
22802011 22802267 30752 32070 + cds coding_transcript
22802011 22802267 30752 32070 + exon coding_transcript
22802011 22802267 30752 32070 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 31006 31065 + cds 2xTY1 CLC
1548 2264 31072 31788 + cds SGFP CLC
2271 2312 31795 31836 + cds V5 CLC
2313 2336 31837 31860 + cds Precision cut site CLC
2337 2357 31861 31881 + cds TEV CLC
2358 2429 31882 31953 + cds BLRP CLC
3813 3846 31961 31994 + misc_recomb FRT CLC

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG5521[31005]::S000169_fly_pretag_rev
GGGAATGGGTGCAGGTAGTACCAGGAATCGGAGGAAAGGGACACGTTTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG5521[31005]::S000169_fly_pretag_fwd
GCGGTGACACGCCGCCCGAGAGCCCAACATTGCCGCAGCGACGCTTCAAAgaagtgcataccaatcaggacccgc
fasta Fasta