Clone: 7730547358441285 H09

Tagged gene: Cdc6
Identifier: FBgn0035918

Clone FlyFos029155(pRedFlp-Hgr)(Cdc6[39067]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
8640245 8644111 19942 23808 - gene CG6486 coding_transcript
8640367 8642036 22017 23686 + gene Arr2 coding_transcript
8638012 8639918 24135 26041 + gene CG13305 coding_transcript
8633574 8636684 27369 30479 - gene Cpr66D coding_transcript
8617473 8631773 32280 47642 - gene Zasp66 coding_transcript
8624609 8627181 36872 40506 + gene Cdc6 coding_transcript
8624609 8627181 36872 40506 + mrna FBtr0076647 coding_transcript
8625480 8627181 36872 38573 + exon coding_transcript
8627050 8627181 36872 37003 + five_prime_utr coding_transcript
8625480 8627049 37004 38573 + cds coding_transcript
8625402 8625479 38574 38651 + intron coding_transcript
8624609 8625401 38652 40506 + exon coding_transcript
8624983 8625401 38652 40132 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 39068 39127 + cds 2xTY1 CLC
1548 2264 39134 39850 + cds SGFP CLC
2271 2312 39857 39898 + cds V5 CLC
2313 2336 39899 39922 + cds Precision cut site CLC
2337 2357 39923 39943 + cds TEV CLC
2358 2429 39944 40015 + cds BLRP CLC
3813 3846 40023 40056 + misc_recomb FRT CLC
dmel-5.43-3L
8624609 8624982 40133 40506 + three_prime_utr coding_transcript
8615139 8616990 48125 49976 + gene GAPsec coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Cdc6[39067]::S000169_fly_pretag_fwd
ACAAGCAGCTGATCGCCTCGATCCTCAGCGATACCGCCTGCCTGAGCAAGgaagtgcataccaatcaggacccgc
 fasta Fasta
Cdc6[39067]::S000169_fly_pretag_rev
GCGGTTTGTCGGGCACACTCATTTCCTAGAGGTAATCGTCCAGATCGTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta