Clone: 7730547358441285 C06

Tagged gene: unc-45
Identifier: FBgn0010812

Clone FlyFos029091(pRedFlp-Hgr)(unc-45[23291]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
3875395 3875781 9734 10120 - gene CG43061 coding_transcript
3874889 3875178 10337 10626 - gene CG43618 coding_transcript
3859027 3862374 23141 27550 - gene unc-45 coding_transcript
3859027 3862374 23141 27550 - mrna FBtr0081788 coding_transcript
3860662 3862374 23141 25915 - exon coding_transcript
3862227 3862374 23141 23288 - three_prime_utr coding_transcript
3860662 3862226 23289 25915 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 23365 23398 - misc_recomb FRT CLC
2358 2429 23406 23477 - cds BLRP CLC
2337 2357 23478 23498 - cds TEV CLC
2313 2336 23499 23522 - cds Precision cut site CLC
2271 2312 23523 23564 - cds V5 CLC
1548 2264 23571 24287 - cds SGFP CLC
1482 1541 24294 24353 - cds 2xTY1 CLC
dmel-5.43-3R
3860603 3860661 25916 25974 - intron coding_transcript
3859310 3860602 25975 27267 - exon coding_transcript
3859324 3860602 25975 27253 - cds coding_transcript
3859310 3859323 27254 27267 - five_prime_utr coding_transcript
3859253 3859309 27268 27324 - intron coding_transcript
3859027 3859252 27325 27550 - exon coding_transcript
3859027 3859252 27325 27550 - five_prime_utr coding_transcript
3857861 3858669 27908 28716 + gene CG11035 coding_transcript
3856133 3857867 28710 30444 - gene CG10903 coding_transcript
3841932 3856505 30072 44645 + gene CG2747 coding_transcript
3853932 3856167 30410 32645 + gene ImpE3 coding_transcript
3839546 3842332 44245 47031 - gene Pbp95 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
unc-45[23291]::S000169_fly_pretag_rev
GAAGATGAAATGGAGGAAAAACAAAAGGCACTACAAACGAATTTCGCTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
unc-45[23291]::S000169_fly_pretag_fwd
TCCCCGATGTGTTTGCCGAAAATTCGAAAATATCTGAGATTATCGATGATgaagtgcataccaatcaggacccgc
 fasta Fasta