Clone: 7409496615490294 C01

Tagged gene: TotB
Identifier: FBgn0038838

Clone FlyFos028209(pRedFlp-Hgr)(TotB[16985]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
16703458 16704164 12994 13700 - gene TotZ coding_transcript
16699672 16700259 16899 18548 - gene TotB coding_transcript
16699672 16700259 16899 18548 - mrna FBtr0083973 coding_transcript
16699782 16700259 16899 18438 - exon coding_transcript
16700176 16700259 16899 16982 - three_prime_utr coding_transcript
16699782 16700175 16983 18438 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 17059 17092 - misc_recomb FRT CLC
2358 2429 17100 17171 - cds BLRP CLC
2337 2357 17172 17192 - cds TEV CLC
2313 2336 17193 17216 - cds Precision cut site CLC
2271 2312 17217 17258 - cds V5 CLC
1548 2264 17265 17981 - cds SGFP CLC
1482 1541 17988 18047 - cds 2xTY1 CLC
dmel-5.43-3R
16699723 16699781 18439 18497 - intron coding_transcript
16699672 16699722 18498 18548 - exon coding_transcript
16699700 16699722 18498 18520 - cds coding_transcript
16699672 16699699 18521 18548 - five_prime_utr coding_transcript
16698710 16699310 18910 19510 - gene TotC coding_transcript
16696758 16697427 20793 21462 - gene TotA coding_transcript
16685904 16691951 26269 32316 + gene CG3822 coding_transcript
16677428 16685667 32553 40792 + gene Ir93a coding_transcript
16676271 16677156 41064 41949 - gene RpS30 coding_transcript
16672833 16673372 44848 45387 - gene CG15696 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
TotB[16985]::S000169_fly_pretag_fwd
TTGCAAGCATATTAACTAGCTGGTTTCCAGCTAACACAGAACGAAACCATgaagtgcataccaatcaggacccgc
 fasta Fasta
TotB[16985]::S000169_fly_pretag_rev
ACTTTCAGTGGGGAATTGATTTTATTACAAAAAAGATGTCTACGATTTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta