Clone: 7270297670695668 C12

Tagged gene: Oatp33Eb
Identifier: FBgn0032435

Clone FlyFos028201(pRedFlp-Hgr)(Oatp33Eb[17863]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
12451945 12453230 16558 17843 + gene CG5418 coding_transcript
12448486 12452053 17735 22364 - gene Oatp33Eb coding_transcript
12448486 12452053 17735 22364 - mrna FBtr0080370 coding_transcript
12451194 12452053 17735 19656 - exon coding_transcript
12451928 12452053 17735 17860 - three_prime_utr coding_transcript
12451194 12451927 17861 19656 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 17937 17970 - misc_recomb FRT CLC
2358 2429 17978 18049 - cds BLRP CLC
2337 2357 18050 18070 - cds TEV CLC
2313 2336 18071 18094 - cds Precision cut site CLC
2271 2312 18095 18136 - cds V5 CLC
1548 2264 18143 18859 - cds SGFP CLC
1482 1541 18866 18925 - cds 2xTY1 CLC
dmel-5.43-2L
12451130 12451193 19657 19720 - intron coding_transcript
12449832 12451129 19721 21018 - exon coding_transcript
12449832 12451129 19721 21018 - cds coding_transcript
12449754 12449831 21019 21096 - intron coding_transcript
12449597 12449753 21097 21253 - exon coding_transcript
12449597 12449753 21097 21253 - cds coding_transcript
12448996 12449596 21254 21854 - intron coding_transcript
12448807 12448995 21855 22043 - exon coding_transcript
12448807 12448995 21855 22043 - cds coding_transcript
12448729 12448806 22044 22121 - intron coding_transcript
12448486 12448728 22122 22364 - exon coding_transcript
12448662 12448728 22122 22188 - cds coding_transcript
12448486 12448661 22189 22364 - five_prime_utr coding_transcript
12446224 12448113 22737 24626 + gene CG5421 coding_transcript
12441475 12445922 24928 29375 + gene Oatp33Ea coding_transcript
12434432 12440605 30245 36418 - gene Elf coding_transcript
12431075 12434665 36185 39775 + gene SC35 coding_transcript
12429827 12430892 39958 41023 + gene CG5435 coding_transcript
12428032 12430021 40829 42818 - gene CG6388 coding_transcript
12426899 12427663 43187 43951 + gene CG5446 coding_transcript
12424904 12426440 44410 45946 - gene CG6405 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Oatp33Eb[17863]::S000169_fly_pretag_fwd
ATCAACCGCAACCTGAGAGTCCGAGGCCACAGAGCCCAGAAACGGACTTCgaagtgcataccaatcaggacccgc
 fasta Fasta
Oatp33Eb[17863]::S000169_fly_pretag_rev
TTTACGAATGTAATAAAAACATATTAGAATACTTTGTGTGACCCCTTTTActtgtcgtcgtcatccttgtagtca
 fasta Fasta