Clone: 3622867014928135 H03

Tagged gene: CG15414
Identifier: FBgn0031542

Clone FlyFos026882(pRedFlp-Hgr)(CG15414[32521]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
3480755 3491048 22430 33785 + gene CG15414 coding_transcript
3480755 3491048 22430 33785 + mrna FBtr0077568 coding_transcript
3490832 3491048 22430 22646 + exon coding_transcript
3490832 3491048 22430 22646 + five_prime_utr coding_transcript
3481917 3490831 22647 31561 + intron coding_transcript
3480755 3481916 31562 33785 + exon coding_transcript
3480954 3481916 31562 33586 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 32522 32581 + cds 2xTY1 CLC
1548 2264 32588 33304 + cds SGFP CLC
2271 2312 33311 33352 + cds V5 CLC
2313 2336 33353 33376 + cds Precision cut site CLC
2337 2357 33377 33397 + cds TEV CLC
2358 2429 33398 33469 + cds BLRP CLC
3813 3846 33477 33510 + misc_recomb FRT CLC
dmel-5.43-2L
3480755 3480953 33587 33785 + three_prime_utr coding_transcript
3478434 3479612 34928 36106 - gene Thor coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG15414[32521]::S000169_fly_pretag_fwd
ACTTCGGTCATGGTCCGCTTGGCGGAGTGGACAACCTGCTGAGGTTCAAGgaagtgcataccaatcaggacccgc
 fasta Fasta
CG15414[32521]::S000169_fly_pretag_rev
CATAGACGTAGGTGCGGATGTGTGCGCGGACAAGGAAGGTAGCCTCTCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta