Clone: 3622867014928135 F03

Tagged gene: mRpL46
Identifier: FBgn0035272

Clone FlyFos026881(pRedFlp-Hgr)(mRpL46[22634]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
1777441 1778347 22577 24545 - gene mRpL46 coding_transcript
1777441 1778347 22577 24545 - mrna FBtr0072776 coding_transcript
1777441 1778347 22577 24545 - exon coding_transcript
1778293 1778347 22577 22631 - three_prime_utr coding_transcript
1777516 1778292 22632 24470 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 22708 22741 - misc_recomb FRT CLC
2358 2429 22749 22820 - cds BLRP CLC
2337 2357 22821 22841 - cds TEV CLC
2313 2336 22842 22865 - cds Precision cut site CLC
2271 2312 22866 22907 - cds V5 CLC
1548 2264 22914 23630 - cds SGFP CLC
1482 1541 23637 23696 - cds 2xTY1 CLC
dmel-5.43-3L
1777441 1777515 24471 24545 - five_prime_utr coding_transcript
1777008 1777363 24623 24978 + gene CG2021 coding_transcript
1775633 1777024 24962 26353 - gene CG12018 coding_transcript
1771809 1775143 26843 30177 + gene CG13933 coding_transcript
1759172 1771650 30336 42814 + gene Dhc62B coding_transcript
1756159 1759208 42778 45827 - gene CG8001 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
mRpL46[22634]::S000169_fly_pretag_rev
TATTTTTATTTATTTGTTAGATATCAAAACAATTTTAACAATTACGATTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
mRpL46[22634]::S000169_fly_pretag_fwd
AAAAGCTGACCAATACAGCCTATGCCCAAAGCGTTAAAAAGTTCTTGTTGgaagtgcataccaatcaggacccgc
 fasta Fasta