Clone: 5835759330954416 D11

Tagged gene: Ubi-p5E
Identifier: FBgn0086558

Clone FlyFos026807(pRedFlp-Hgr)(Ubi-p5E[39336]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
6198023 6200778 9738 12493 - gene CG3823 coding_transcript
6193084 6195303 15213 17432 + gene CG12219 coding_transcript
6189424 6193006 17510 21092 - gene CG3815 coding_transcript
6187137 6189148 21368 23379 + gene CG15892 coding_transcript
6187137 6189148 21368 23379 + gene CG15891 coding_transcript
6185058 6187186 23330 25458 - gene CG15896 coding_transcript
6183503 6185276 25240 27013 + gene Rpt4 coding_transcript
6182156 6183595 26921 28360 - gene wuho coding_transcript
6178504 6182027 28489 32012 + gene Top3beta coding_transcript
6171010 6173305 37211 40568 + gene Ubi-p5E coding_transcript
6171010 6173305 37211 40568 + mrna FBtr0070933 coding_transcript
6173169 6173305 37211 37347 + exon coding_transcript
6173169 6173305 37211 37347 + five_prime_utr coding_transcript
6172787 6173168 37348 37729 + intron coding_transcript
6171010 6172786 37730 40568 + exon coding_transcript
6172782 6172786 37730 37734 + five_prime_utr coding_transcript
6171177 6172781 37735 40401 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 39337 39396 + cds 2xTY1 CLC
1548 2264 39403 40119 + cds SGFP CLC
2271 2312 40126 40167 + cds V5 CLC
2313 2336 40168 40191 + cds Precision cut site CLC
2337 2357 40192 40212 + cds TEV CLC
2358 2429 40213 40284 + cds BLRP CLC
3813 3846 40292 40325 + misc_recomb FRT CLC
dmel-5.43-X
6171010 6171176 40402 40568 + three_prime_utr coding_transcript
6169005 6170745 40833 42573 + gene CG3566 coding_transcript
6161847 6169019 42559 49731 - gene Spt6 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Ubi-p5E[39336]::S000169_fly_pretag_rev
CCTGTGGCACAACTAACTAACTTCAATTTTTATAAGGGTGAAAAAATCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta
Ubi-p5E[39336]::S000169_fly_pretag_fwd
AGAAGGAGTCGACCCTTCACTTGGTCCTGCGTCTGCGTGGTGGCGCCATCgaagtgcataccaatcaggacccgc
 fasta Fasta