Clone: 5835759330954416 B06
Tagged gene: Scsalpha
Identifier: FBgn0004888
| Clone | FlyFos026706(pRedFlp-Hgr)(Scsalpha[21225]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT |
|---|---|
| Library | FlyFos 5.43 D.mel in E. coli EPI300 |
| Genome | Species: Drosophila melanogaster, Release: 5.43 |
GenBank
GFF
Validation(click to hide details)
| Type | Status | Download / View |
|---|---|---|
| Grows in selection | good | |
| NGS-based tag validation | bad |
Genes in pFlyFos vector(click to hide details)
| Gene | Clone relative | Genome relative | |||||
|---|---|---|---|---|---|---|---|
| Name | Length | Start | End | Strand | Start | End | Strand |
| DsRed | 716 | 8417 | 9132 | + | 8417 | 9132 | + |
Features(click to show details)
| Start (Source) | End (Source) | Start | End | Strand | Type | Name | Source | |
|---|---|---|---|---|---|---|---|---|
| pFlyFos | ||||||||
| 1 | 107 | 1 | 107 | - | cds | LacZ | epicentre | |
| 4 | 21 | 4 | 21 | - | primer_bind | CC2_rev | epicentre | |
| 310 | 593 | 310 | 593 | + | misc_recomb | attB | epicentre | |
| 727 | 1386 | 727 | 1386 | - | cds | Cat | epicentre | |
| 1605 | 1952 | 1605 | 1952 | + | cds | redF | epicentre | |
| 2336 | 2953 | 2336 | 2953 | + | rep_origin | oriV | epicentre | |
| 2953 | 3007 | 2953 | 3007 | + | rep_origin | ori2 | epicentre | |
| 3347 | 4102 | 3347 | 4102 | + | cds | repE | epicentre | |
| 4681 | 5856 | 4681 | 5856 | + | cds | parA | epicentre | |
| 5856 | 6827 | 5856 | 6827 | + | cds | parB | epicentre | |
| 6900 | 7417 | 6900 | 7417 | + | repeat_region | parC | epicentre | |
| 7633 | 8031 | 7633 | 8031 | + | misc_binding | cos | epicentre | |
| 8049 | 8082 | 8049 | 8082 | + | misc_recomb | loxP | epicentre | |
| 8182 | 8416 | 8182 | 8416 | + | promoter | 3xP3 | epicentre | |
| 8417 | 9132 | 8417 | 9132 | + | gene | DsRed | epicentre | |
| 8452 | 9132 | 8452 | 9132 | + | cds | DsRed | epicentre | |
| 9284 | 9334 | 9284 | 9334 | + | terminator | SV40 | epicentre | |
| 9408 | 9630 | 9408 | 9630 | - | cds | LacZ | epicentre | |
| 9559 | 9576 | 9559 | 9576 | + | primer_bind | T7 | epicentre | |
| 9610 | 9627 | 9610 | 9627 | + | primer_bind | CC2_fwd | epicentre | |
| dmel-5.43-3L | ||||||||
| 3806714 | 3809090 | 9943 | 12319 | + | gene | IntS10 | coding_transcript | |
| 3809146 | 3810181 | 12375 | 13410 | - | gene | CG32262 | coding_transcript | |
| 3810312 | 3811114 | 13541 | 14343 | - | gene | CG32263 | coding_transcript | |
| 3811269 | 3813958 | 14498 | 17187 | + | gene | CG12006 | coding_transcript | |
| 3814190 | 3818383 | 17419 | 22674 | + | gene | Scsalpha | coding_transcript | |
| 3814190 | 3818383 | 17419 | 22674 | + | mrna | FBtr0073151 | coding_transcript | |
| 3814190 | 3818154 | 17419 | 22445 | + | mrna | FBtr0303096 | coding_transcript | |
| 3814190 | 3814355 | 17419 | 17584 | + | exon | coding_transcript | ||
| 3814190 | 3814355 | 17419 | 17584 | + | exon | coding_transcript | ||
| 3814190 | 3814318 | 17419 | 17547 | + | five_prime_utr | coding_transcript | ||
| 3814190 | 3814318 | 17419 | 17547 | + | five_prime_utr | coding_transcript | ||
| 3814319 | 3814355 | 17548 | 17584 | + | cds | coding_transcript | ||
| 3814319 | 3814355 | 17548 | 17584 | + | cds | coding_transcript | ||
| 3814356 | 3816631 | 17585 | 19860 | + | intron | coding_transcript | ||
| 3814356 | 3816631 | 17585 | 19860 | + | intron | coding_transcript | ||
| 3815923 | 3816354 | 19152 | 19583 | - | gene | Acp63F | coding_transcript | |
| 3815923 | 3816354 | 19152 | 19583 | - | mrna | FBtr0073182 | coding_transcript | |
| 3815923 | 3816046 | 19152 | 19275 | - | exon | coding_transcript | ||
| 3815923 | 3815984 | 19152 | 19213 | - | three_prime_utr | coding_transcript | ||
| 3815985 | 3816046 | 19214 | 19275 | - | cds | coding_transcript | ||
| 3816047 | 3816100 | 19276 | 19329 | - | intron | coding_transcript | ||
| 3816101 | 3816256 | 19330 | 19485 | - | exon | coding_transcript | ||
| 3816101 | 3816256 | 19330 | 19485 | - | cds | coding_transcript | ||
| 3816257 | 3816317 | 19486 | 19546 | - | intron | coding_transcript | ||
| 3816318 | 3816354 | 19547 | 19583 | - | exon | coding_transcript | ||
| 3816318 | 3816345 | 19547 | 19574 | - | cds | coding_transcript | ||
| 3816346 | 3816354 | 19575 | 19583 | - | five_prime_utr | coding_transcript | ||
| 3816632 | 3817080 | 19861 | 20309 | + | exon | coding_transcript | ||
| 3816632 | 3817080 | 19861 | 20309 | + | cds | coding_transcript | ||
| 3816632 | 3817080 | 19861 | 20309 | + | exon | coding_transcript | ||
| 3816632 | 3817080 | 19861 | 20309 | + | cds | coding_transcript | ||
| 3817081 | 3817436 | 20310 | 20665 | + | intron | coding_transcript | ||
| 3817081 | 3817436 | 20310 | 20665 | + | intron | coding_transcript | ||
| 3817437 | 3817721 | 20666 | 20950 | + | exon | coding_transcript | ||
| 3817437 | 3817721 | 20666 | 20950 | + | cds | coding_transcript | ||
| 3817437 | 3817721 | 20666 | 20950 | + | exon | coding_transcript | ||
| 3817437 | 3817721 | 20666 | 20950 | + | cds | coding_transcript | ||
| 3817722 | 3817783 | 20951 | 21012 | + | intron | coding_transcript | ||
| 3817722 | 3817783 | 20951 | 21012 | + | intron | coding_transcript | ||
| 3817784 | 3818383 | 21013 | 22674 | + | exon | coding_transcript | ||
| 3817784 | 3818154 | 21013 | 22445 | + | exon | coding_transcript | ||
| 3817784 | 3817999 | 21013 | 22290 | + | cds | coding_transcript | ||
| 3817784 | 3817999 | 21013 | 22290 | + | cds | coding_transcript | ||
| p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG | ||||||||
| 1482 | 1541 | 21226 | 21285 | + | cds | 2xTY1 | CLC | |
| 1548 | 2264 | 21292 | 22008 | + | cds | SGFP | CLC | |
| 2271 | 2312 | 22015 | 22056 | + | cds | V5 | CLC | |
| 2313 | 2336 | 22057 | 22080 | + | cds | Precision cut site | CLC | |
| 2337 | 2357 | 22081 | 22101 | + | cds | TEV | CLC | |
| 2358 | 2429 | 22102 | 22173 | + | cds | BLRP | CLC | |
| 3813 | 3846 | 22181 | 22214 | + | misc_recomb | FRT | CLC | |
| dmel-5.43-3L | ||||||||
| 3818000 | 3818383 | 22291 | 22674 | + | three_prime_utr | coding_transcript | ||
| 3818000 | 3818154 | 22291 | 22445 | + | three_prime_utr | coding_transcript | ||
| 3825449 | 3828525 | 29740 | 32816 | + | gene | Rdh | coding_transcript | |
Sets(click to hide details)
Oligos(click to hide details)
| Name/Sequence | Export |
|---|---|
|
Scsalpha[21225]::S000169_fly_pretag_rev
GCTTTGATCGGCGTACGGATTCCGGTTTGGTTATGGCCTTTAATTATTTActtgtcgtcgtcatccttgtagtca
|
Fasta
|
|
Scsalpha[21225]::S000169_fly_pretag_fwd
CCAAAATGGGCCACGAGCTCTTCAAGGAGATGAAGCGTCTGGAGTTAGTGgaagtgcataccaatcaggacccgc
|
Fasta
|