Clone: 03996259806504221 A08

Tagged gene: l(1)G0144
Identifier: FBgn0027296

Clone FlyFos026539(pRedFlp-Hgr)(l(1)G0144[24854]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
2165318 2169111 10473 14266 + gene CG2918 coding_transcript
2159024 2164789 14795 20560 + gene CG2924 coding_transcript
2156873 2158858 20726 22711 - gene CG3071 coding_transcript
2154236 2156560 23024 26410 + gene l(1)G0144 coding_transcript
2154236 2156560 23024 26410 + mrna FBtr0070414 coding_transcript
2155984 2156560 23024 23600 + exon coding_transcript
2156070 2156560 23024 23514 + five_prime_utr coding_transcript
2155984 2156069 23515 23600 + cds coding_transcript
2155888 2155983 23601 23696 + intron coding_transcript
2155499 2155887 23697 24085 + exon coding_transcript
2155499 2155887 23697 24085 + cds coding_transcript
2155449 2155498 24086 24135 + intron coding_transcript
2154236 2155448 24136 26410 + exon coding_transcript
2154727 2155448 24136 25919 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 24855 24914 + cds 2xTY1 CLC
1548 2264 24921 25637 + cds SGFP CLC
2271 2312 25644 25685 + cds V5 CLC
2313 2336 25686 25709 + cds Precision cut site CLC
2337 2357 25710 25730 + cds TEV CLC
2358 2429 25731 25802 + cds BLRP CLC
3813 3846 25810 25843 + misc_recomb FRT CLC
dmel-5.43-X
2154236 2154726 25920 26410 + three_prime_utr coding_transcript
2148432 2153896 26750 32214 + gene CG3078 coding_transcript
2146365 2148167 32479 34281 + gene CG3091 coding_transcript
2144178 2146026 34620 36468 + gene CG3191 coding_transcript
2141627 2144015 36631 39019 + gene crn coding_transcript
2140264 2141609 39037 40382 - gene Or2a coding_transcript
2136137 2140079 40567 44509 - gene fs(1)K10 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
l(1)G0144[24854]::S000169_fly_pretag_fwd
AGCACCGGCGGTGGTGCAATCTTCACCTCAGCTTTGGATATCCAGCGGACgaagtgcataccaatcaggacccgc
fasta Fasta
l(1)G0144[24854]::S000169_fly_pretag_rev
TAATTCTATTGGGGTAGATTGATTTGAGCTTTATCAGGTCGCGCTGTCTActtgtcgtcgtcatccttgtagtca
fasta Fasta