Clone: 03996259806504221 H06

Tagged gene: Sirt4
Identifier: FBgn0029783

Clone FlyFos026518(pRedFlp-Hgr)(Sirt4[32903]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
5547551 5556085 21690 30224 - gene CG42265 coding_transcript
5558661 5560002 32800 35203 - gene Sirt4 coding_transcript
5558661 5560002 32800 35203 - mrna FBtr0070850 coding_transcript
5558661 5560002 32800 35203 - mrna FBtr0070851 coding_transcript
5558661 5560002 32800 35203 - mrna FBtr0070852 coding_transcript
5558661 5559660 32800 34861 - exon coding_transcript
5558661 5559519 32800 34720 - exon coding_transcript
5558661 5559519 32800 34720 - exon coding_transcript
5558661 5558761 32800 32900 - three_prime_utr coding_transcript
5558661 5558761 32800 32900 - three_prime_utr coding_transcript
5558661 5558761 32800 32900 - three_prime_utr coding_transcript
5558762 5559519 32901 34720 - cds coding_transcript
5558762 5559451 32901 34652 - cds coding_transcript
5558762 5559451 32901 34652 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 32977 33010 - misc_recomb FRT CLC
2358 2429 33018 33089 - cds BLRP CLC
2337 2357 33090 33110 - cds TEV CLC
2313 2336 33111 33134 - cds Precision cut site CLC
2271 2312 33135 33176 - cds V5 CLC
1548 2264 33183 33899 - cds SGFP CLC
1482 1541 33906 33965 - cds 2xTY1 CLC
dmel-5.43-X
5559452 5559660 34653 34861 - five_prime_utr coding_transcript
5559452 5559519 34653 34720 - five_prime_utr coding_transcript
5559520 5559720 34721 34921 - intron coding_transcript
5559520 5559642 34721 34843 - intron coding_transcript
5559643 5559660 34844 34861 - exon coding_transcript
5559643 5559660 34844 34861 - five_prime_utr coding_transcript
5559661 5559720 34862 34921 - intron coding_transcript
5559661 5559720 34862 34921 - intron coding_transcript
5559721 5560002 34922 35203 - exon coding_transcript
5559721 5560002 34922 35203 - five_prime_utr coding_transcript
5559721 5560002 34922 35203 - exon coding_transcript
5559721 5560002 34922 35203 - five_prime_utr coding_transcript
5559721 5560002 34922 35203 - exon coding_transcript
5559721 5559901 34922 35102 - cds coding_transcript
5559902 5560002 35103 35203 - five_prime_utr coding_transcript
5560127 5564586 35328 39787 + gene CG4119 coding_transcript
5564860 5565842 40061 41043 - gene Rab-RP4 coding_transcript
5566475 5567576 41676 42777 + gene RpL35 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Sirt4[32903]::S000169_fly_pretag_rev
CCATAACTACAAATTTAGTGGGGGTCAACATATAACAACTGTGAGTATTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Sirt4[32903]::S000169_fly_pretag_fwd
GCGATGTCATACCCAAACTGTTCGACTTTCGCAATTCGAAAAGCGTCAGTgaagtgcataccaatcaggacccgc
fasta Fasta