Clone: 03996259806504221 G05

Tagged gene: TwdlB
Identifier: FBgn0039436

Clone FlyFos026514(pRedFlp-Hgr)(TwdlB[22498]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
22459456 22460437 10354 11335 - gene TwdlH coding_transcript
22457595 22458680 12111 13196 - gene TwdlN coding_transcript
22455678 22456502 14289 15113 - gene TwdlJ coding_transcript
22453686 22454429 16362 17105 + gene TwdlK coding_transcript
22451730 22452419 18372 19061 + gene TwdlO coding_transcript
22450302 22451374 19417 20489 + gene TwdlL coding_transcript
22448173 22449270 21521 23680 + gene TwdlB coding_transcript
22448173 22449270 21521 23680 + mrna FBtr0085057 coding_transcript
22449204 22449270 21521 21587 + exon coding_transcript
22449219 22449270 21521 21572 + five_prime_utr coding_transcript
22449204 22449218 21573 21587 + cds coding_transcript
22449136 22449203 21588 21655 + intron coding_transcript
22448173 22449135 21656 23680 + exon coding_transcript
22448290 22449135 21656 23563 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 22499 22558 + cds 2xTY1 CLC
1548 2264 22565 23281 + cds SGFP CLC
2271 2312 23288 23329 + cds V5 CLC
2313 2336 23330 23353 + cds Precision cut site CLC
2337 2357 23354 23374 + cds TEV CLC
2358 2429 23375 23446 + cds BLRP CLC
3813 3846 23454 23487 + misc_recomb FRT CLC
dmel-5.43-3R
22448173 22448289 23564 23680 + three_prime_utr coding_transcript
22446330 22447082 24771 25523 + gene TwdlP coding_transcript
22444845 22445953 25900 27008 - gene TwdlM coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
TwdlB[22498]::S000169_fly_pretag_rev
TTCAGTCGAGATTTGCTTAAGTTAATGGAGTCCAAGATGTAGCCTTCCTActtgtcgtcgtcatccttgtagtca
fasta Fasta
TwdlB[22498]::S000169_fly_pretag_fwd
TCCCCGACAACGCCTACCTGCCCACTTCTGTGTTCCGTCGTCTGCGTTTCgaagtgcataccaatcaggacccgc
fasta Fasta