Clone: 03996259806504221 F05

Tagged gene: TwdlJ
Identifier: FBgn0039440

Clone FlyFos026514(pRedFlp-Hgr)(TwdlJ[14291]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
22459456 22460437 10354 11335 - gene TwdlH coding_transcript
22457595 22458680 12111 13196 - gene TwdlN coding_transcript
22455678 22456502 14289 16175 - gene TwdlJ coding_transcript
22455678 22456502 14289 16175 - mrna FBtr0114538 coding_transcript
22455678 22456502 14289 16175 - exon coding_transcript
22455678 22456502 14289 16175 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 14365 14398 - misc_recomb FRT CLC
2358 2429 14406 14477 - cds BLRP CLC
2337 2357 14478 14498 - cds TEV CLC
2313 2336 14499 14522 - cds Precision cut site CLC
2271 2312 14523 14564 - cds V5 CLC
1548 2264 14571 15287 - cds SGFP CLC
1482 1541 15294 15353 - cds 2xTY1 CLC
dmel-5.43-3R
22453686 22454429 17424 18167 + gene TwdlK coding_transcript
22451730 22452419 19434 20123 + gene TwdlO coding_transcript
22450302 22451374 20479 21551 + gene TwdlL coding_transcript
22448173 22449270 22583 23680 + gene TwdlB coding_transcript
22446330 22447082 24771 25523 + gene TwdlP coding_transcript
22444845 22445953 25900 27008 - gene TwdlM coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
TwdlJ[14291]::S000169_fly_pretag_rev
TTATTTCGTATTATAATCTCAACAATAACAAAGGAAATGCCAGGAACTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
TwdlJ[14291]::S000169_fly_pretag_fwd
CCCCAGGTAGCTCCTACTTGCCCGCCAACATCCTGCGCCGCCTGCGTTTCgaagtgcataccaatcaggacccgc
fasta Fasta