Clone: 03996259806504221 F04

Tagged gene: CG12655
Identifier: FBgn0031080

Clone FlyFos026504(pRedFlp-Hgr)(CG12655[35543]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
19852426 19853421 35434 37491 - gene CG12655 coding_transcript
19852426 19853421 35434 37491 - mrna FBtr0070036 coding_transcript
19852426 19852933 35434 37003 - exon coding_transcript
19852426 19852532 35434 35540 - three_prime_utr coding_transcript
19852533 19852933 35541 37003 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 35617 35650 - misc_recomb FRT CLC
2358 2429 35658 35729 - cds BLRP CLC
2337 2357 35730 35750 - cds TEV CLC
2313 2336 35751 35774 - cds Precision cut site CLC
2271 2312 35775 35816 - cds V5 CLC
1548 2264 35823 36539 - cds SGFP CLC
1482 1541 36546 36605 - cds 2xTY1 CLC
dmel-5.43-X
19852934 19853333 37004 37403 - intron coding_transcript
19853334 19853421 37404 37491 - exon coding_transcript
19853334 19853400 37404 37470 - cds coding_transcript
19853401 19853421 37471 37491 - five_prime_utr coding_transcript
19855330 19862712 39400 46782 + gene Nep3 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG12655[35543]::S000169_fly_pretag_rev
AGAGACTAATTCACAAAAAAAAAAAACGATCCCACACTGCAACCGCATCActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG12655[35543]::S000169_fly_pretag_fwd
GCGTCACCGTGACCATAGCACTTGGATTGGCCTTGGGCGGCATGAGATTTgaagtgcataccaatcaggacccgc
fasta Fasta