Clone: 03996259806504221 E04

Tagged gene: Nep3
Identifier: FBgn0031081

Clone FlyFos026504(pRedFlp-Hgr)(Nep3[44498]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
19852426 19853421 35434 36429 - gene CG12655 coding_transcript
19855330 19862712 38338 46782 + gene Nep3 coding_transcript
19855330 19862712 38338 46782 + mrna FBtr0307555 coding_transcript
19855330 19855633 38338 38641 + exon coding_transcript
19855330 19855633 38338 38641 + five_prime_utr coding_transcript
19855634 19857987 38642 40995 + intron coding_transcript
19855722 19862512 38730 46582 + mrna FBtr0070000 coding_transcript
19855722 19855878 38730 38886 + exon coding_transcript
19855722 19855878 38730 38886 + five_prime_utr coding_transcript
19855879 19857987 38887 40995 + intron coding_transcript
19857209 19862512 40217 46582 + mrna FBtr0307554 coding_transcript
19857209 19857374 40217 40382 + exon coding_transcript
19857209 19857374 40217 40382 + five_prime_utr coding_transcript
19857375 19857987 40383 40995 + intron coding_transcript
19857988 19858104 40996 41112 + exon coding_transcript
19857988 19858104 40996 41112 + cds coding_transcript
19857988 19858104 40996 41112 + exon coding_transcript
19857988 19858104 40996 41112 + cds coding_transcript
19857988 19858104 40996 41112 + exon coding_transcript
19857988 19858104 40996 41112 + cds coding_transcript
19858105 19858814 41113 41822 + intron coding_transcript
19858105 19858814 41113 41822 + intron coding_transcript
19858105 19858814 41113 41822 + intron coding_transcript
19858815 19858977 41823 41985 + exon coding_transcript
19858815 19858977 41823 41985 + cds coding_transcript
19858815 19858977 41823 41985 + exon coding_transcript
19858815 19858977 41823 41985 + cds coding_transcript
19858815 19858977 41823 41985 + exon coding_transcript
19858815 19858977 41823 41985 + cds coding_transcript
19858978 19859038 41986 42046 + intron coding_transcript
19858978 19859038 41986 42046 + intron coding_transcript
19858978 19859038 41986 42046 + intron coding_transcript
19859039 19859159 42047 42167 + exon coding_transcript
19859039 19859159 42047 42167 + cds coding_transcript
19859039 19859159 42047 42167 + exon coding_transcript
19859039 19859159 42047 42167 + cds coding_transcript
19859039 19859159 42047 42167 + exon coding_transcript
19859039 19859159 42047 42167 + cds coding_transcript
19859160 19859229 42168 42237 + intron coding_transcript
19859160 19859229 42168 42237 + intron coding_transcript
19859160 19859229 42168 42237 + intron coding_transcript
19859230 19859544 42238 42552 + exon coding_transcript
19859230 19859544 42238 42552 + cds coding_transcript
19859230 19859544 42238 42552 + exon coding_transcript
19859230 19859544 42238 42552 + cds coding_transcript
19859230 19859544 42238 42552 + exon coding_transcript
19859230 19859544 42238 42552 + cds coding_transcript
19859545 19859609 42553 42617 + intron coding_transcript
19859545 19859609 42553 42617 + intron coding_transcript
19859545 19859609 42553 42617 + intron coding_transcript
19859610 19860104 42618 43112 + exon coding_transcript
19859610 19860104 42618 43112 + cds coding_transcript
19859610 19860104 42618 43112 + exon coding_transcript
19859610 19860104 42618 43112 + cds coding_transcript
19859610 19860104 42618 43112 + exon coding_transcript
19859610 19860104 42618 43112 + cds coding_transcript
19860105 19860215 43113 43223 + intron coding_transcript
19860105 19860215 43113 43223 + intron coding_transcript
19860105 19860215 43113 43223 + intron coding_transcript
19860216 19861045 43224 44053 + exon coding_transcript
19860216 19861045 43224 44053 + cds coding_transcript
19860216 19861045 43224 44053 + exon coding_transcript
19860216 19861045 43224 44053 + cds coding_transcript
19860216 19861045 43224 44053 + exon coding_transcript
19860216 19861045 43224 44053 + cds coding_transcript
19861046 19861113 44054 44121 + intron coding_transcript
19861046 19861113 44054 44121 + intron coding_transcript
19861046 19861113 44054 44121 + intron coding_transcript
19861114 19861256 44122 44264 + exon coding_transcript
19861114 19861256 44122 44264 + cds coding_transcript
19861114 19861256 44122 44264 + exon coding_transcript
19861114 19861256 44122 44264 + cds coding_transcript
19861114 19861256 44122 44264 + exon coding_transcript
19861114 19861256 44122 44264 + cds coding_transcript
19861257 19861316 44265 44324 + intron coding_transcript
19861257 19861316 44265 44324 + intron coding_transcript
19861257 19861316 44265 44324 + intron coding_transcript
19861317 19862712 44325 46782 + exon coding_transcript
19861317 19862512 44325 46582 + exon coding_transcript
19861317 19862512 44325 46582 + exon coding_transcript
19861317 19861493 44325 45563 + cds coding_transcript
19861317 19861493 44325 45563 + cds coding_transcript
19861317 19861493 44325 45563 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 44499 44558 + cds 2xTY1 CLC
1548 2264 44565 45281 + cds SGFP CLC
2271 2312 45288 45329 + cds V5 CLC
2313 2336 45330 45353 + cds Precision cut site CLC
2337 2357 45354 45374 + cds TEV CLC
2358 2429 45375 45446 + cds BLRP CLC
3813 3846 45454 45487 + misc_recomb FRT CLC
dmel-5.43-X
19861494 19862712 45564 46782 + three_prime_utr coding_transcript
19861494 19862512 45564 46582 + three_prime_utr coding_transcript
19861494 19862512 45564 46582 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Nep3[44498]::S000169_fly_pretag_rev
TTGGCTCCATCTTTGGCTCGTTCTGAGATCGAAATTGCACTATCCCCTTActtgtcgtcgtcatccttgtagtca
fasta Fasta
Nep3[44498]::S000169_fly_pretag_fwd
AGTGCAAGCCCGGCAAACGGATGAATCCCACTGAGAAGTGCGAGGTGTGGgaagtgcataccaatcaggacccgc
fasta Fasta