Clone: 03996259806504221 G03

Tagged gene: CG33543
Identifier: FBgn0053543

Clone FlyFos026492(pRedFlp-Hgr)(CG33543[41986]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
2008460 2008966 30797 31303 - gene Nplp4 coding_transcript
2006763 2007193 32570 33000 + gene CG15353 coding_transcript
1997102 2006307 33456 43723 + gene CG33543 coding_transcript
1997102 2006307 33456 43723 + mrna FBtr0309285 coding_transcript
2005561 2006307 33456 34202 + exon coding_transcript
2005616 2006307 33456 34147 + five_prime_utr coding_transcript
2005561 2005615 34148 34202 + cds coding_transcript
2002921 2005560 34203 36842 + intron coding_transcript
2002669 2002920 36843 37094 + exon coding_transcript
2002669 2002920 36843 37094 + cds coding_transcript
2002314 2002668 37095 37449 + intron coding_transcript
2002173 2002313 37450 37590 + exon coding_transcript
2002173 2002313 37450 37590 + cds coding_transcript
2001512 2002172 37591 38251 + intron coding_transcript
2001380 2001511 38252 38383 + exon coding_transcript
2001380 2001511 38252 38383 + cds coding_transcript
2001087 2001379 38384 38676 + intron coding_transcript
2000934 2001086 38677 38829 + exon coding_transcript
2000934 2001086 38677 38829 + cds coding_transcript
1998721 2000933 38830 41042 + intron coding_transcript
1998232 1998720 41043 41531 + exon coding_transcript
1998232 1998720 41043 41531 + cds coding_transcript
1997959 1998231 41532 41804 + intron coding_transcript
1997102 1997958 41805 43723 + exon coding_transcript
1997774 1997958 41805 43051 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 41987 42046 + cds 2xTY1 CLC
1548 2264 42053 42769 + cds SGFP CLC
2271 2312 42776 42817 + cds V5 CLC
2313 2336 42818 42841 + cds Precision cut site CLC
2337 2357 42842 42862 + cds TEV CLC
2358 2429 42863 42934 + cds BLRP CLC
3813 3846 42942 42975 + misc_recomb FRT CLC
dmel-5.43-2L
1997102 1997773 43052 43723 + three_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG33543[41986]::S000169_fly_pretag_fwd
GTCCTTGGCTGTATCCGTCATATGGACTCATTCTCGCTTTGATCTGGACAgaagtgcataccaatcaggacccgc
fasta Fasta
CG33543[41986]::S000169_fly_pretag_rev
TGGGTTGGGATTGCATTTGGTATTGGTGATCGGTATAGGTCGAGGCTCTActtgtcgtcgtcatccttgtagtca
fasta Fasta