Clone: 03996259806504221 B01

Tagged gene: CG32971
Identifier: FBgn0052971

Clone FlyFos026452(pRedFlp-Hgr)(CG32971[24961]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
14274795 14278002 24874 29143 - gene CG32971 coding_transcript
14274795 14278002 24874 29143 - mrna FBtr0114462 coding_transcript
14274795 14278002 24874 29143 - mrna FBtr0114463 coding_transcript
14277211 14278002 24874 26727 - mrna FBtr0080621 coding_transcript
14277313 14278002 24874 26625 - exon coding_transcript
14277462 14278002 24874 26476 - exon coding_transcript
14277462 14278002 24874 26476 - exon coding_transcript
14277918 14278002 24874 24958 - three_prime_utr coding_transcript
14277918 14278002 24874 24958 - three_prime_utr coding_transcript
14277918 14278002 24874 24958 - three_prime_utr coding_transcript
14277480 14277917 24959 26458 - cds coding_transcript
14277480 14277917 24959 26458 - cds coding_transcript
14277480 14277917 24959 26458 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 25035 25068 - misc_recomb FRT CLC
2358 2429 25076 25147 - cds BLRP CLC
2337 2357 25148 25168 - cds TEV CLC
2313 2336 25169 25192 - cds Precision cut site CLC
2271 2312 25193 25234 - cds V5 CLC
1548 2264 25241 25957 - cds SGFP CLC
1482 1541 25964 26023 - cds 2xTY1 CLC
dmel-5.43-2L
14277313 14277479 26459 26625 - five_prime_utr coding_transcript
14277462 14277479 26459 26476 - five_prime_utr coding_transcript
14277462 14277479 26459 26476 - five_prime_utr coding_transcript
14277401 14277461 26477 26537 - intron coding_transcript
14277401 14277461 26477 26537 - intron coding_transcript
14277211 14277400 26538 26727 - exon coding_transcript
14277211 14277400 26538 26727 - five_prime_utr coding_transcript
14277313 14277400 26538 26625 - exon coding_transcript
14277313 14277400 26538 26625 - five_prime_utr coding_transcript
14274941 14277312 26626 28997 - intron coding_transcript
14274941 14277312 26626 28997 - intron coding_transcript
14274795 14274940 28998 29143 - exon coding_transcript
14274795 14274940 28998 29143 - five_prime_utr coding_transcript
14274795 14274940 28998 29143 - exon coding_transcript
14274795 14274940 28998 29143 - five_prime_utr coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG32971[24961]::S000169_fly_pretag_fwd
ACTACGAAAAGAGCGGTGGACCTGACTTGGATTCAAAGGATTTACTTGTTgaagtgcataccaatcaggacccgc
fasta Fasta
CG32971[24961]::S000169_fly_pretag_rev
TACAGTGGTTATCCTTTACGAATGGATAAAAAATATATCATTTATAATTActtgtcgtcgtcatccttgtagtca
fasta Fasta