Clone: 8900262712670307 C10

Tagged gene: Mgstl
Identifier: FBgn0025814

Clone FlyFos026416(pRedFlp-Hgr)(Mgstl[16860]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
20910541 20911632 12371 13462 - gene CG15449 coding_transcript
20913516 20915134 15346 18026 + gene Mgstl coding_transcript
20913516 20915134 15346 18026 + mrna FBtr0077242 coding_transcript
20913516 20913674 15346 15504 + exon coding_transcript
20913516 20913545 15346 15375 + five_prime_utr coding_transcript
20913546 20913674 15376 15504 + cds coding_transcript
20913675 20914706 15505 16536 + intron coding_transcript
20914099 20915134 15929 18026 + mrna FBtr0077243 coding_transcript
20914099 20914328 15929 16158 + exon coding_transcript
20914099 20914196 15929 16026 + five_prime_utr coding_transcript
20914197 20914328 16027 16158 + cds coding_transcript
20914329 20914706 16159 16536 + intron coding_transcript
20914707 20915134 16537 18026 + exon coding_transcript
20914707 20915134 16537 18026 + exon coding_transcript
20914707 20915033 16537 17925 + cds coding_transcript
20914707 20915033 16537 17925 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 16861 16920 + cds 2xTY1 CLC
1548 2264 16927 17643 + cds SGFP CLC
2271 2312 17650 17691 + cds V5 CLC
2313 2336 17692 17715 + cds Precision cut site CLC
2337 2357 17716 17736 + cds TEV CLC
2358 2429 17737 17808 + cds BLRP CLC
3813 3846 17816 17849 + misc_recomb FRT CLC
dmel-5.43-X
20915034 20915134 17926 18026 + three_prime_utr coding_transcript
20915034 20915134 17926 18026 + three_prime_utr coding_transcript
20916165 20920127 19057 23019 + gene Cbs coding_transcript
20920991 20925143 23883 28035 - gene CG1518 coding_transcript
20933411 20938560 36303 41452 - gene CG32512 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Mgstl[16860]::S000169_fly_pretag_fwd
TGGGCGCCACCGTCTACATGGCCCTGCAGGTCATCGCCTCGGCCGCCTTCgaagtgcataccaatcaggacccgc
fasta Fasta
Mgstl[16860]::S000169_fly_pretag_rev
TTTCAAAATGCTTAAAAAAAAAAACAAGAAGGACTAGACCTATGTGCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta