Clone: 8900262712670307 G09

Tagged gene: Cyp6a22
Identifier: FBgn0013773

Clone FlyFos026410(pRedFlp-Hgr)(Cyp6a22[19092]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2R
10766861 10768877 11087 13103 + gene Cyp6a9 coding_transcript
10765293 10767257 12707 14671 - gene Cyp6a19 coding_transcript
10763338 10765159 14805 16626 - gene Cyp6a23 coding_transcript
10761459 10763063 16901 18505 - gene Cyp6a17 coding_transcript
10759178 10760970 18994 21848 - gene Cyp6a22 coding_transcript
10759178 10760970 18994 21848 - mrna FBtr0087450 coding_transcript
10760441 10760970 18994 20585 - exon coding_transcript
10760875 10760970 18994 19089 - three_prime_utr coding_transcript
10760441 10760874 19090 20585 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19166 19199 - misc_recomb FRT CLC
2358 2429 19207 19278 - cds BLRP CLC
2337 2357 19279 19299 - cds TEV CLC
2313 2336 19300 19323 - cds Precision cut site CLC
2271 2312 19324 19365 - cds V5 CLC
1548 2264 19372 20088 - cds SGFP CLC
1482 1541 20095 20154 - cds 2xTY1 CLC
dmel-5.43-2R
10760374 10760440 20586 20652 - intron coding_transcript
10759178 10760373 20653 21848 - exon coding_transcript
10759308 10760373 20653 21718 - cds coding_transcript
10759178 10759307 21719 21848 - five_prime_utr coding_transcript
10748661 10758575 22451 32365 + gene Pcf11 coding_transcript
10746350 10748250 32776 34676 + gene U3-55K coding_transcript
10744952 10746138 34888 36074 + gene CG33506 coding_transcript
10742947 10745007 36019 38079 - gene HPS1 coding_transcript
10741368 10742573 38453 39658 + gene Ciao1 coding_transcript
10740470 10741453 39573 40556 - gene Ercc1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Cyp6a22[19092]::S000169_fly_pretag_fwd
CCGCCAACGGAATTTACTTAAAAGTCCAACAGCTTAGCCAAATGAGTAGTgaagtgcataccaatcaggacccgc
 fasta Fasta
Cyp6a22[19092]::S000169_fly_pretag_rev
ATACTAAATGTTAGAATACAATCCAGATAGCGATTTAATAAGCAGCACTActtgtcgtcgtcatccttgtagtca
 fasta Fasta