Clone: 14365767356821757 G12

Tagged gene: wisp
Identifier: FBgn0260780

Clone FlyFos025915(pRedFlp-Hgr)(wisp[27558]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-X
11781313 11782277 15370 16334 + gene SelG coding_transcript
11782391 11783222 16448 17279 + gene CG1840 coding_transcript
11786043 11787782 20100 21839 + gene CG15738 coding_transcript
11787978 11793943 22035 29062 + gene wisp coding_transcript
11787978 11793943 22035 29062 + mrna FBtr0073573 coding_transcript
11787978 11788227 22035 22284 + exon coding_transcript
11787978 11788227 22035 22284 + five_prime_utr coding_transcript
11788228 11788295 22285 22352 + intron coding_transcript
11788296 11788584 22353 22641 + exon coding_transcript
11788296 11788584 22353 22641 + five_prime_utr coding_transcript
11788585 11788777 22642 22834 + intron coding_transcript
11788778 11789852 22835 23909 + exon coding_transcript
11788778 11788796 22835 22853 + five_prime_utr coding_transcript
11788797 11789852 22854 23909 + cds coding_transcript
11789853 11789909 23910 23966 + intron coding_transcript
11789910 11791396 23967 25453 + exon coding_transcript
11789910 11791396 23967 25453 + cds coding_transcript
11791397 11791464 25454 25521 + intron coding_transcript
11791465 11791933 25522 25990 + exon coding_transcript
11791465 11791933 25522 25990 + cds coding_transcript
11791934 11791986 25991 26043 + intron coding_transcript
11791987 11792200 26044 26257 + exon coding_transcript
11791987 11792200 26044 26257 + cds coding_transcript
11792201 11792256 26258 26313 + intron coding_transcript
11792257 11792400 26314 26457 + exon coding_transcript
11792257 11792400 26314 26457 + cds coding_transcript
11792401 11792688 26458 26745 + intron coding_transcript
11792689 11792998 26746 27055 + exon coding_transcript
11792689 11792998 26746 27055 + cds coding_transcript
11792999 11793062 27056 27119 + intron coding_transcript
11793063 11793943 27120 29062 + exon coding_transcript
11793063 11793504 27120 28623 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 27559 27618 + cds 2xTY1 CLC
1548 2264 27625 28341 + cds SGFP CLC
2271 2312 28348 28389 + cds V5 CLC
2313 2336 28390 28413 + cds Precision cut site CLC
2337 2357 28414 28434 + cds TEV CLC
2358 2429 28435 28506 + cds BLRP CLC
3813 3846 28514 28547 + misc_recomb FRT CLC
dmel-5.43-X
11793505 11793943 28624 29062 + three_prime_utr coding_transcript
11794470 11799373 29589 34492 - gene Upf1 coding_transcript
11800485 11800846 35604 35965 - gene CG43156 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
wisp[27558]::S000169_fly_pretag_rev
AAAGTTGTTGTACTTTCTTTTTATGTTTGCCATTTAAAGGGCTACAATTActtgtcgtcgtcatccttgtagtca
fasta Fasta
wisp[27558]::S000169_fly_pretag_fwd
ACCCAGATGCAGCGTCGACCTTTGCCGAAACGACTGCAGCGCATGTCGCCgaagtgcataccaatcaggacccgc
fasta Fasta