Clone: 14365767356821757 D12

Tagged gene: Ir25a
Identifier: FBgn0031634

Clone FlyFos025911(pRedFlp-Hgr)(Ir25a[21986]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]
Whole tag sequencing full download [show/hide details]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-2L
4821776 4828552 12792 19568 + gene CG15628 coding_transcript
4829381 4830686 20397 21702 - gene CG2976 coding_transcript
4830846 4835300 21862 27378 - gene Ir25a coding_transcript
4830846 4835300 21862 27378 - mrna FBtr0289979 coding_transcript
4830846 4831361 21862 23439 - exon coding_transcript
4830846 4830967 21862 21983 - three_prime_utr coding_transcript
4830968 4831361 21984 23439 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 22060 22093 - misc_recomb FRT CLC
2358 2429 22101 22172 - cds BLRP CLC
2337 2357 22173 22193 - cds TEV CLC
2313 2336 22194 22217 - cds Precision cut site CLC
2271 2312 22218 22259 - cds V5 CLC
1548 2264 22266 22982 - cds SGFP CLC
1482 1541 22989 23048 - cds 2xTY1 CLC
dmel-5.43-2L
4831362 4831416 23440 23494 - intron coding_transcript
4831417 4831932 23495 24010 - exon coding_transcript
4831417 4831932 23495 24010 - cds coding_transcript
4831933 4832003 24011 24081 - intron coding_transcript
4832004 4832596 24082 24674 - exon coding_transcript
4832004 4832596 24082 24674 - cds coding_transcript
4832597 4832794 24675 24872 - intron coding_transcript
4832795 4833040 24873 25118 - exon coding_transcript
4832795 4833040 24873 25118 - cds coding_transcript
4833041 4833101 25119 25179 - intron coding_transcript
4833102 4833216 25180 25294 - exon coding_transcript
4833102 4833216 25180 25294 - cds coding_transcript
4833217 4833265 25295 25343 - intron coding_transcript
4833266 4833704 25344 25782 - exon coding_transcript
4833266 4833704 25344 25782 - cds coding_transcript
4833705 4833768 25783 25846 - intron coding_transcript
4833769 4834044 25847 26122 - exon coding_transcript
4833769 4834044 25847 26122 - cds coding_transcript
4834045 4834106 26123 26184 - intron coding_transcript
4834107 4834256 26185 26334 - exon coding_transcript
4834107 4834256 26185 26334 - cds coding_transcript
4834257 4834873 26335 26951 - intron coding_transcript
4834874 4834972 26952 27050 - exon coding_transcript
4834874 4834949 26952 27027 - cds coding_transcript
4834950 4834972 27028 27050 - five_prime_utr coding_transcript
4834973 4835246 27051 27324 - intron coding_transcript
4835247 4835300 27325 27378 - exon coding_transcript
4835247 4835300 27325 27378 - five_prime_utr coding_transcript
4835333 4837996 27411 30074 - gene CG15626 coding_transcript
4839219 4841465 31297 33543 + gene CG12194 coding_transcript
4842086 4847292 34164 39370 - gene CG2950 coding_transcript
4847748 4849499 39826 41577 + gene CG11927 coding_transcript
4849439 4850418 41517 42496 - gene mRpS2 coding_transcript
4850639 4852640 42717 44718 + gene CG11926 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
Ir25a[21986]::S000169_fly_pretag_fwd
CACTGCGTCCGCGCTTTAATCAGTATCCGGCCACGTTTAAGCCTCGTTTCgaagtgcataccaatcaggacccgc
fasta Fasta
Ir25a[21986]::S000169_fly_pretag_rev
AGTGCTCATTTTACCATAATATTCGTTTCATTGGTATACGAGTAATTCTActtgtcgtcgtcatccttgtagtca
fasta Fasta