Clone: 14365767356821757 B07

Tagged gene: TfIIEalpha
Identifier: FBgn0015828

Clone FlyFos025862(pRedFlp-Hgr)(TfIIEalpha[24220]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
11675126 11676905 22580 25421 + gene TfIIEalpha coding_transcript
11675126 11676905 22580 25421 + mrna FBtr0076108 coding_transcript
11675126 11675322 22580 22776 + exon coding_transcript
11675126 11675322 22580 22776 + five_prime_utr coding_transcript
11675323 11675400 22777 22854 + intron coding_transcript
11675401 11676507 22855 23961 + exon coding_transcript
11675401 11675411 22855 22865 + five_prime_utr coding_transcript
11675412 11676507 22866 23961 + cds coding_transcript
11676508 11676575 23962 24029 + intron coding_transcript
11676576 11676905 24030 25421 + exon coding_transcript
11676576 11676769 24030 25285 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 24221 24280 + cds 2xTY1 CLC
1548 2264 24287 25003 + cds SGFP CLC
2271 2312 25010 25051 + cds V5 CLC
2313 2336 25052 25075 + cds Precision cut site CLC
2337 2357 25076 25096 + cds TEV CLC
2358 2429 25097 25168 + cds BLRP CLC
3813 3846 25176 25209 + misc_recomb FRT CLC
dmel-5.43-3L
11676770 11676905 25286 25421 + three_prime_utr coding_transcript
11677084 11683255 25600 31771 + gene Sug coding_transcript
11682731 11684499 31247 33015 - gene pallidin coding_transcript
11684747 11685857 33263 34373 + gene CG14135 coding_transcript
11686979 11690086 35495 38602 - gene CG11652 coding_transcript
11690226 11692363 38742 40879 - gene CG6053 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
TfIIEalpha[24220]::S000169_fly_pretag_fwd
AGGAGAACTACATACACGTATACCAGCAGCACTACAGCCATATATTCGAAgaagtgcataccaatcaggacccgc
fasta Fasta
TfIIEalpha[24220]::S000169_fly_pretag_rev
GGGAAACTCGTGGAAAACGTAGAAATCGTGGCTAATTGCAGACGCCCTCActtgtcgtcgtcatccttgtagtca
fasta Fasta