Clone: 14365767356821757 B05

Tagged gene: CG4476
Identifier: FBgn0035969

Clone FlyFos025832(pRedFlp-Hgr)(CG4476[19881]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation good [view]

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
9204984 9208369 19702 24149 - gene CG4476 coding_transcript
9204984 9208369 19702 24149 - mrna FBtr0076429 coding_transcript
9207936 9208369 19702 21197 - exon coding_transcript
9208193 9208369 19702 19878 - three_prime_utr coding_transcript
9207936 9208192 19879 21197 - cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
2437 2470 19955 19988 - misc_recomb FRT CLC
2358 2429 19996 20067 - cds BLRP CLC
2337 2357 20068 20088 - cds TEV CLC
2313 2336 20089 20112 - cds Precision cut site CLC
2271 2312 20113 20154 - cds V5 CLC
1548 2264 20161 20877 - cds SGFP CLC
1482 1541 20884 20943 - cds 2xTY1 CLC
dmel-5.43-3L
9207871 9207935 21198 21262 - intron coding_transcript
9207694 9207870 21263 21439 - exon coding_transcript
9207694 9207870 21263 21439 - cds coding_transcript
9207563 9207693 21440 21570 - intron coding_transcript
9206498 9207562 21571 22635 - exon coding_transcript
9206498 9207562 21571 22635 - cds coding_transcript
9206301 9206497 22636 22832 - intron coding_transcript
9205973 9206300 22833 23160 - exon coding_transcript
9205973 9206300 22833 23160 - cds coding_transcript
9205831 9205972 23161 23302 - intron coding_transcript
9205756 9205830 23303 23377 - exon coding_transcript
9205768 9205830 23303 23365 - cds coding_transcript
9205756 9205767 23366 23377 - five_prime_utr coding_transcript
9205314 9205755 23378 23819 - intron coding_transcript
9204984 9205313 23820 24149 - exon coding_transcript
9204984 9205313 23820 24149 - five_prime_utr coding_transcript
9196165 9201953 27180 32968 + gene Slc45-1 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG4476[19881]::S000169_fly_pretag_fwd
ATGTTAATCGCCGTTGTGTTTGCTACACAGCTGGAGATCGAATTTTCGGTgaagtgcataccaatcaggacccgc
fasta Fasta
CG4476[19881]::S000169_fly_pretag_rev
TGCAAAGTTTTTGAGGTGACATAAAATAATATAAAAGTAAGTTTGGTTTActtgtcgtcgtcatccttgtagtca
fasta Fasta