Clone: 8180008395487431 B04

Tagged gene: CG5114
Identifier: FBgn0036460

Clone FlyFos025615(pRedFlp-Hgr)(CG5114[19803]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good
NGS-based tag validation bad

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
14967948 14969140 10230 11422 + gene Ocho coding_transcript
14972738 14975443 15020 17725 + gene CG3349 coding_transcript
14975948 14977586 18230 20930 + gene CG5114 coding_transcript
14975948 14977586 18230 20930 + mrna FBtr0075624 coding_transcript
14975948 14977586 18230 20930 + mrna FBtr0309064 coding_transcript
14975948 14976950 18230 19232 + exon coding_transcript
14975948 14976890 18230 19172 + exon coding_transcript
14975948 14976034 18230 18316 + five_prime_utr coding_transcript
14975948 14976034 18230 18316 + five_prime_utr coding_transcript
14976035 14976950 18317 19232 + cds coding_transcript
14976035 14976890 18317 19172 + cds coding_transcript
14976891 14977012 19173 19294 + intron coding_transcript
14976951 14977012 19233 19294 + intron coding_transcript
14977013 14977220 19295 19502 + exon coding_transcript
14977013 14977220 19295 19502 + cds coding_transcript
14977013 14977220 19295 19502 + exon coding_transcript
14977013 14977220 19295 19502 + cds coding_transcript
14977221 14977310 19503 19592 + intron coding_transcript
14977221 14977310 19503 19592 + intron coding_transcript
14977311 14977586 19593 20930 + exon coding_transcript
14977311 14977586 19593 20930 + exon coding_transcript
14977311 14977524 19593 20868 + cds coding_transcript
14977311 14977524 19593 20868 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 19804 19863 + cds 2xTY1 CLC
1548 2264 19870 20586 + cds SGFP CLC
2271 2312 20593 20634 + cds V5 CLC
2313 2336 20635 20658 + cds Precision cut site CLC
2337 2357 20659 20679 + cds TEV CLC
2358 2429 20680 20751 + cds BLRP CLC
3813 3846 20759 20792 + misc_recomb FRT CLC
dmel-5.43-3L
14977525 14977586 20869 20930 + three_prime_utr coding_transcript
14977525 14977586 20869 20930 + three_prime_utr coding_transcript
14978215 14984767 21559 28111 + gene mnd coding_transcript
14985389 14990917 28733 34261 + gene CG10006 coding_transcript
14991081 14992764 34425 36108 - gene mRpL39 coding_transcript
14993036 14994419 36380 37763 + gene Prosbeta2 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG5114[19803]::S000169_fly_pretag_rev
TTTACTATTTACATATATATAGCACAGTACAAAACTTTAAAACCTAACTActtgtcgtcgtcatccttgtagtca
fasta Fasta
CG5114[19803]::S000169_fly_pretag_fwd
TATCCGAGGACAACACGGCCAAGATATTCAAGGTGCCGGCGCTGGGCGATgaagtgcataccaatcaggacccgc
fasta Fasta