Clone: 6536462692990512 D09

Tagged gene: CG5447
Identifier: FBgn0039427

Clone FlyFos025428(pRedFlp-Hgr)(CG5447[35308]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3R
22267049 22274716 22929 30596 + gene tx coding_transcript
22274801 22276307 30681 32187 + gene Hex-t1 coding_transcript
22276374 22278049 32254 33929 + gene Hex-t2 coding_transcript
22278256 22280307 34136 37249 + gene CG5447 coding_transcript
22278256 22280307 34136 37249 + mrna FBtr0085012 coding_transcript
22278256 22278379 34136 34259 + exon coding_transcript
22278256 22278379 34136 34259 + five_prime_utr coding_transcript
22278380 22278575 34260 34455 + intron coding_transcript
22278576 22278744 34456 34624 + exon coding_transcript
22278576 22278582 34456 34462 + five_prime_utr coding_transcript
22278583 22278744 34463 34624 + cds coding_transcript
22278745 22278810 34625 34690 + intron coding_transcript
22278811 22278963 34691 34843 + exon coding_transcript
22278811 22278963 34691 34843 + cds coding_transcript
22278964 22279290 34844 35170 + intron coding_transcript
22279291 22279630 35171 36572 + exon coding_transcript
22279291 22279431 35171 36373 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 35309 35368 + cds 2xTY1 CLC
1548 2264 35375 36091 + cds SGFP CLC
2271 2312 36098 36139 + cds V5 CLC
2313 2336 36140 36163 + cds Precision cut site CLC
2337 2357 36164 36184 + cds TEV CLC
2358 2429 36185 36256 + cds BLRP CLC
3813 3846 36264 36297 + misc_recomb FRT CLC
dmel-5.43-3R
22279432 22279630 36374 36572 + three_prime_utr coding_transcript
22279631 22279701 36573 36643 + intron coding_transcript
22279702 22280307 36644 37249 + exon coding_transcript
22279702 22280307 36644 37249 + three_prime_utr coding_transcript
22280535 22280762 37477 37704 + gene CG43117 coding_transcript
22281184 22281863 38126 38805 - gene Pdf coding_transcript
22281858 22282964 38800 39906 + gene CG14237 coding_transcript
22284075 22286469 41017 43411 + gene CG14238 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
CG5447[35308]::S000169_fly_pretag_rev
GCTCGATCTCACATGAAAAACTCGTTGGCTGAATGGGAAAGCGGAAGTCActtgtcgtcgtcatccttgtagtca
 fasta Fasta
CG5447[35308]::S000169_fly_pretag_fwd
GGGGACTTCGCGTCATAGAGATTACAATATTCGATCGTCCGGGGCGAACGgaagtgcataccaatcaggacccgc
 fasta Fasta