Clone: 6536462692990512 E07

Tagged gene: eIF4E-3
Identifier: FBgn0035860

Clone FlyFos025403(pRedFlp-Hgr)(eIF4E-3[23156]::S000169_fly_pretag)::2XTY1-SGFP-V5-preTEV-BLRP-3XFLAGdFRT
Library FlyFos 5.43 D.mel in E. coli EPI300
Genome Species: Drosophila melanogaster, Release: 5.43

Validation(click to hide details)

Type Status Download / View
Grows in selection good

Genes in pFlyFos vector(click to hide details)

Gene Clone relative Genome relative
Name Length Start End Strand Start End Strand
DsRed 716 8417 9132 + 8417 9132 +

Features(click to show details)

Start (Source) End (Source) Start End Strand Type Name Source
pFlyFos
1 107 1 107 - cds LacZ epicentre
4 21 4 21 - primer_bind CC2_rev epicentre
310 593 310 593 + misc_recomb attB epicentre
727 1386 727 1386 - cds Cat epicentre
1605 1952 1605 1952 + cds redF epicentre
2336 2953 2336 2953 + rep_origin oriV epicentre
2953 3007 2953 3007 + rep_origin ori2 epicentre
3347 4102 3347 4102 + cds repE epicentre
4681 5856 4681 5856 + cds parA epicentre
5856 6827 5856 6827 + cds parB epicentre
6900 7417 6900 7417 + repeat_region parC epicentre
7633 8031 7633 8031 + misc_binding cos epicentre
8049 8082 8049 8082 + misc_recomb loxP epicentre
8182 8416 8182 8416 + promoter 3xP3 epicentre
8417 9132 8417 9132 + gene DsRed epicentre
8452 9132 8452 9132 + cds DsRed epicentre
9284 9334 9284 9334 + terminator SV40 epicentre
9408 9630 9408 9630 - cds LacZ epicentre
9559 9576 9559 9576 + primer_bind T7 epicentre
9610 9627 9610 9627 + primer_bind CC2_fwd epicentre
dmel-5.43-3L
8211332 8212083 10970 11721 - gene CG13674 coding_transcript
8215243 8216053 14881 15691 + gene CG13678 coding_transcript
8222333 8223668 21971 24368 + gene eIF4E-3 coding_transcript
8222333 8223668 21971 24368 + mrna FBtr0076701 coding_transcript
8222333 8222533 21971 22171 + exon coding_transcript
8222333 8222481 21971 22119 + five_prime_utr coding_transcript
8222482 8222533 22120 22171 + cds coding_transcript
8222534 8222598 22172 22236 + intron coding_transcript
8222599 8222857 22237 22495 + exon coding_transcript
8222599 8222857 22237 22495 + cds coding_transcript
8222858 8222923 22496 22561 + intron coding_transcript
8222924 8222998 22562 22636 + exon coding_transcript
8222924 8222998 22562 22636 + cds coding_transcript
8222999 8223052 22637 22690 + intron coding_transcript
8223053 8223152 22691 22790 + exon coding_transcript
8223053 8223152 22691 22790 + cds coding_transcript
8223153 8223215 22791 22853 + intron coding_transcript
8223216 8223303 22854 22941 + exon coding_transcript
8223216 8223303 22854 22941 + cds coding_transcript
8223304 8223360 22942 22998 + intron coding_transcript
8223361 8223668 22999 24368 + exon coding_transcript
8223361 8223521 22999 24221 + cds coding_transcript
p2XTY1-SGFP-V5-preTEV-BLRP-3XFLAG
1482 1541 23157 23216 + cds 2xTY1 CLC
1548 2264 23223 23939 + cds SGFP CLC
2271 2312 23946 23987 + cds V5 CLC
2313 2336 23988 24011 + cds Precision cut site CLC
2337 2357 24012 24032 + cds TEV CLC
2358 2429 24033 24104 + cds BLRP CLC
3813 3846 24112 24145 + misc_recomb FRT CLC
dmel-5.43-3L
8223522 8223668 24222 24368 + three_prime_utr coding_transcript
8224914 8233701 25614 34401 - gene CG7213 coding_transcript

Sets(click to hide details)

Oligos(click to hide details)

Name/Sequence Export
eIF4E-3[23156]::S000169_fly_pretag_fwd
AGGATGCCATGGTGAACCATGGACCCAATGTCAATGCCATCTACACATTGgaagtgcataccaatcaggacccgc
 fasta Fasta
eIF4E-3[23156]::S000169_fly_pretag_rev
TTATGTACTATCGTTGGAAATGTACTATGGCGCCCTTGGAAATTGGCCTActtgtcgtcgtcatccttgtagtca
 fasta Fasta